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N1408

Sigma-Aldrich

Nerve Growth Factor β Human

>97% (SDS-PAGE), recombinant, expressed in NSO cells, lyophilized powder, suitable for cell culture

Synonyme(s) :

HSAN5, NGFB, hNGFβ

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About This Item

Numéro MDL:
MFCD00164982
Code UNSPSC :
12352202
Nomenclature NACRES :
NA.77

product name

NGF-β human, from human, recombinant, expressed in NSO cells, lyophilized powder, suitable for cell culture

Source biologique

human

Niveau de qualité

200

Produit recombinant

expressed in NSO cells

Pureté

>97% (SDS-PAGE)

Forme

lyophilized powder

Puissance

0.15-3.0 ng/mL ED50/EC50

Qualité

endotoxin tested

Conditionnement

pkg of 5X0.1 mg
pkg of 0.1 mg

Technique(s)

cell culture | mammalian: suitable

Impuretés

<1 EU/μg

Solubilité

water: soluble 0.1 g/L at 20 °C

Numéro d'accès UniProt

P01138

Température de stockage

−20°C

Informations sur le gène

human ... NGF(4803)

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Description générale

The nerve growth factor β (NGFB) gene is mapped to human chromosome 1p13.2.

Application

NGF-β human has been used:
  • in protein pin arrays
  • to induce U87-MG (glioblastoma cell line) cell proliferation
  • in drug release kinetics

Actions biochimiques/physiologiques

Nerve growth factor β (NGFB) acts as a key mediator in neuronal and non-neuronal development, differentiation, and survival. Polymorphism of NGFB gene leads to hereditary sensory and autonomic neuropathies. Charcot arthropathy and thermal insensitivity are few of the related symptoms. NGFβ is known to promote cancer cell proliferation and metastasis, also mediates angiogenesis. It facilitates the development of various cancer including, oral, breast, lungs, pancreatic and prostate cancers. Elevated expression of NGFβ contributes to progression of human cholangiocarcinoma. In animal models, NGFβ is observed to prevent cholinergic neuron loss and enhances memory. NGF-β promotes the survival and cholinergic phenotype of basal forebrain cholinergic neurons and stimulates neurite outgrowth of embryonic trigeminal motor neurons in vitro.

Forme physique

Lyophilized from 0.2% acetic acid containing 5 mg bovine serum albumin.

Remarque sur l'analyse

The biological activity is measured in a cell proliferation assay using a factor-dependent human erythroleukemic cell line, TF-1.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

K A Crutcher et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 13(6), 2540-2550 (1993-06-01)
A two-site ELISA and a bioassay were used to detect NGF-like activity in human brain tissue. Both assays detected mouse NGF and recombinant human NGF with approximately equal sensitivity, whereas the antibodies showed little cross-reactivity with the recombinant human proteins
Nerve Growth Factor Stimulates Glioblastoma Proliferation through Notch1 Receptor Signaling
Park JC, et al.
Journal of Korean Neurosurgical Society, 61(4), 441-441 (2018)
Mahmoud Aarabi et al.
Fertility and sterility, 102(2), 440-447 (2014-06-09)
To determine the levels of postacrosomal WW binding protein (PAWP) in the spermatozoa of men that were used clinically for intracytoplasmic sperm injection (ICSI) and to correlate them with infertility treatment outcomes. Prospective clinical and laboratory study. University-based laboratory and
S Korsching
The Journal of neuroscience : the official journal of the Society for Neuroscience, 13(7), 2739-2748 (1993-07-01)
The neurotrophic factor concept in its basic form envisages that innervated tissues produce a signal for the innervating neurons for the selective limitation of neuronal death occurring during development (Purves, 1986; Oppenheim, 1991). This concept arose several decades ago on
A phase 1 clinical trial of nerve growth factor gene therapy for Alzheimer disease
Tuszynski MH, et al.
Nature Medicine, 11(5), 551-551 (2005)
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