M3769
Medium 199
Modified, with Earle′s salts, without L-glutamine, sodium bicarbonate, and phenol red, powder, suitable for cell culture
Synonyme(s) :
M199 Medium, TCM 199
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About This Item
Code UNSPSC :
12352207
Nomenclature NACRES :
NA.71
Produits recommandés
Niveau de qualité
Forme
powder
Technique(s)
cell culture | mammalian: suitable
Composants
L-glutamine: no
phenol red: no
HEPES: no
sodium pyruvate: no
Earle’s salts (5% CO2): yes
NaHCO3: no
Conditions d'expédition
ambient
Température de stockage
2-8°C
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Description générale
Medium 199 is a combination of vitamins, amino acids, and other factors that were originally developed as a completely defined media formulation for the culture of primary explants. This medium, when properly supplemented has broad applicability, particularly for non-transformed cells. Medium 199 is widely used for vaccine production, in vitro cultivation of primary pancreatic explants, and lens tissues.
Application
Medium 199 has been used:
- to culture the primary human umbilical vein endothelial cells (HUVECs) for use in cell viability assay
- to flush the uterine horn of gilts to recover day 12 conceptuses from uteri
- to culture the peritoneal cells and immortalized human mesothelial cells (MeT-5A and ATCC-CRL-9444)
Quantité
Formulated to contain 9.4 grams of powder per liter of medium.
Reconstitution
Supplement with 0.1 g/L L-glutamine, 2.2 g/L sodium bicarbonate.
Souvent commandé avec ce produit
Réf. du produit
Description
Tarif
Supplément
Code de la classe de stockage
13 - Non Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
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