M3682

Anti-MAP Kinase, Monophosphorylated Tyrosine antibody ,Mouse monoclonal

clone ERK-PY193, purified from hybridoma cell culture

• Synonyme(s) : Anti-pY-ERK
• Numéro MDL: MFCD02096848
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.43

Propriétés

• Source biologique: mouse
• Conjugué: unconjugated
• Forme d'anticorps: purified from hybridoma cell culture
• Type de produit anticorps: primary antibodies
• Clone: ERK-PY193, monoclonal
• Forme: buffered aqueous solution
• Poids mol.: antigen, ERK-1 44 kDa; antigen, ERK-2 42 kDa
• Espèces réactives: rat, human
• Concentration: ~2 mg/mL
• Technique(s): capture ELISA: suitable; immunocytochemistry: suitable; microarray: suitable; western blot: 1-5 μg/mL using cell extract of rat fibroblasts cell line, Rat1, activated with sorbitol
• Isotype: IgG1
• Numéro d'accès UniProt: P28482
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: phosphorylation (pTyr)
• Informations sur le gène: human ... MAPK1(5594) , MAPK3(5595) ; rat ... Mapk1(116590) , Mapk3(50689)

Description

Description générale

Monoclonal Anti-MAP Kinase, Mono-phosphorylated Tyrosine (pY-ERK) (mouse IgG1 isotype) is derived from the ERK-PY193 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide sequence corresponding to the phosphorylated form of ERK-activation loop, conjugated to KLH. The mitogen-activated protein kinase (MAPK) superfamily of enzymes is involved in many signaling pathways. This family includes the ERK1/2 (extracellular signal-regulated protein kinase, also termed p42/p44 MAPK), JNK (c-Jun N-terminal protein kinase, also termed stress-activated protein kinase, SAPK1), and p38 MAPK (also termed SAPK2) subfamilies.

Spécificité

The antibody reacts with the monophosphorylated tyrosine form of MAP kinases (ERK1 and ERK2). It does not recognize the non-phosphorylated, diphosphorylated, and the monophosphorylated threonine forms of ERK/MAP kinases, or the diphosphorylated forms of JNK and p38 MAPK. The epitope recognized by the antibody contains the phosphorylated tyrosine residue within the regulatory site of MAP kinase (e.g.,Tyr¹⁸⁵ in ERK-2). Cross-reactivity has been observed with the monophosphorylated tyrosine peptide of JNK.

Immunogène

synthetic peptide HTGFLTEpYVAT, corresponding to the phosphorylated form of ERK-activation loop.

Application

Monoclonal Anti-MAP Kinase, Monophosphorylated Tyrosine antibody has been used in:

• immunoblotting
• enzyme linked immunosorbent assay (ELISA)
• dot-blot
• immunocytochemistry
• western blotting
• immunofluorescence

Actions biochimiques/physiologiques

MAPK kinase (MAPKK) is the immediate upstream activator of the MAPK, MAPKK kinase (MAP3K), and MAP3K kinase (MAP4K) for the enzymes further upstream, respectively. The kinases in the MAPK level are activated by phosphorylation of both tyrosine (Y) and threonine (T) residues organized in a TXY motif. The residue in between the two phosphorylated residues determines the specificity of activation of the MAPKs. Phosphorylation of both tyrosine and threonine is essential for the full activation of all MAPKs.

Forme physique

Solution in phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Notes préparatoires

Prepared from a culture supernatant of bioreactor grown hybridoma.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable