Accéder au contenu
Merck
Toutes les photos(1)

Key Documents

M2442

Sigma-Aldrich

Anti-phospho-N-Methyl D-Aspartate NR2B Subunit (pTyr1472) antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonyme(s) :

Anti-phospho-NMDA NR2B (pTyr1472)

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme


About This Item

Numéro MDL:
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

rabbit

Niveau de qualité

Conjugué

unconjugated

Forme d'anticorps

affinity isolated antibody

Type de produit anticorps

primary antibodies

Clone

polyclonal

Forme

buffered aqueous solution

Poids mol.

antigen 180 kDa

Espèces réactives

mouse, rat, Xenopus, chicken, human

Technique(s)

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
western blot: 1:1,000 using rat brain (hippocampal) homogenate

Numéro d'accès UniProt

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

phosphorylation (pTyr1472)

Informations sur le gène

human ... GRIN2B(2904)
mouse ... Grin2b(14812)
rat ... Grin2b(24410)

Description générale

Rabbit polyclonal anti-phospho- N-Methyl D-Aspartate NR2B Subunit (pTyr-1472) antibody specifically recognizes rat receptor NR2B subunit (~180 kDa) phosphorylated on tyrosine 1472. It does not crossreact with non-phosphorylated NMDA NR2B. It is likely to detect human, mouse, chicken and Xenopus NMDA NR2B based on sequence homology.
Specifically recognizes the NMDA receptor NR2B subunit phosphorylated on tyrosine 1472. Does not cross-react with non-phosphorylated NMDAR. Two additional lower molecular weight immunoreactive bands are present in immunoblots of rat brain.
The ionotropic glutamate receptor is a prominent ligand-gated and voltage-gated ion channel in excitatory synaptic transmission in the mammalian central nervous system. The glutamate receptor was named the NMDA receptor for its agonist, N-methyl-D-aspartate. The NMDA channel is regulated by its phosphorylation. Functional NMDA receptors contain an active invariant subunit (NMDAR1) associated with one of several variable regulatory subunits. NR2B is one of the variant regulatory subunits associated with the active subunit NMDAR1 and localized primarily to the hippocampus and forebrain. Tyrosine phosphorylation sites on NR2B have been identified. Phosphorylation of tyrosine 1472 on the NR2B subunit is largely dependent on Fyn, a member of the tyrosine kinase oncogene family which regulates long-term potentiation, spatial learning, and hippocampal development, and is elevated upon the long-term potentiation (LTP) induction of hippocampal CA1 neurons. Tyrosine 1472 phosphorylation of NR2B is important for synaptic plasticity.

Immunogène

synthetic phosphorylated peptide derived from the region of NMDA receptor NR2B that is phosphorylated on tyrosine 1472.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Rabbit polyclonal anti-phospho-N-Methyl D-Aspartate NR2B Subunit (pTyr-1472) antibody is used to tag the pTyr1472-NR2B subunit of NMDA for detection and quantitation by immunocytochemical and immunohistochemical (IHC) techniques. It is used as a probe to determine the presence and roles of pTyr1472 phosphorylated NR2B subunit of NMDA in neural functions such as long-term potentiation (LTP) and synaptic plasticity.

Forme physique

Solution in 10 mM HEPES, pH 7.5, and 150 mM NaCl containing 100 μg/ml BSA and 50% glycerol.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Vous ne trouvez pas le bon produit ?  

Essayez notre Outil de sélection de produits.

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Bok Soon Go et al.
European neuropsychopharmacology : the journal of the European College of Neuropsychopharmacology, 26(12), 1989-1999 (2016-10-22)
Cocaine self-administration induces dysfunctional neuroadaptations in the prefrontal cortex that underlie relapse to cocaine-seeking. Cocaine self-administration disturbs glutamatergic transmission in the nucleus accumbens that is prevented by infusion of brain-derived neurotrophic factor (BDNF) into the prelimbic area of the prefrontal
Sílvia Castany et al.
Scientific reports, 8(1), 3873-3873 (2018-03-03)
Sigma-1 receptor (σ1R) knockout (KO) CD1 mice, generated by homologous recombination, and separate pharmacological studies in wild type (WT) mice were done to investigate the role of this receptor in the development of pain-related behaviours (thermal hyperalgesia and mechanical allodynia)
Somi Kim et al.
The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology, 19(6), 515-522 (2015-11-12)
Notch signaling is a key regulator of neuronal fate during embryonic development, but its function in the adult brain is still largely unknown. Mind bomb-2 (Mib2) is an essential positive regulator of the Notch pathway, which acts in the Notch
Natividade de Sá Couto-Pereira et al.
Frontiers in molecular neuroscience, 12, 134-134 (2019-06-14)
Early life experiences program lifelong responses to stress. In agreement, resilience and vulnerability to psychopathologies, such as posttraumatic stress disorder (PTSD), have been suggested to depend on the early background. New therapies have targeted memory reconsolidation as a strategy to
Meng Suo et al.
Journal of neurophysiology, 116(2), 448-455 (2016-05-06)
Diabetic neuropathy is a common complication of diabetes. This study evaluated the role of Fyn kinase and N-methyl-d-aspartate receptors (NMDARs) in the spinal cord in diabetic neuropathy using an animal model of high-fat diet-induced prediabetes. We found that prediabetic wild-type

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique