Accéder au contenu
Merck
Toutes les photos(3)

Principaux documents

Voir toute la documentation

L9504

Sigma-Aldrich

D-Luciferin

luminescent, ≥98% (HPLC), powder

Synonyme(s) :

(S)-2-(6-Hydroxy-2-benzothiazolyl)-2-thiazoline-4-carboxylic acid, 4,5-Dihydro-2-(6-hydroxy-2-benzothiazolyl)-4-thiazolecarboxylic acid, Firefly Luciferin

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
Toutes les photos(3)

About This Item

Formule empirique (notation de Hill) :
C11H8N2O3S2
Numéro CAS:
2591-17-5
Poids moléculaire :
280.32
Beilstein:
30484
Numéro CE :
219-981-3
Numéro MDL:
MFCD00042929
Code UNSPSC :
12352204
ID de substance PubChem :
24896524
Nomenclature NACRES :
NA.32

Nom du produit

D-Luciferin, synthetic

Source biologique

synthetic

Niveau de qualité

300

Essai

≥98% (HPLC)

Forme

powder

Solubilité

DMSO: 10 mg/mL, clear, colorless to faintly yellow

Fluorescence

λex 328 nm; λem 532 nm in H2O

Température de stockage

−20°C

Chaîne SMILES 

OC(=O)[C@H]1CSC(=N1)c2nc3ccc(O)cc3s2

InChI

1S/C11H8N2O3S2/c14-5-1-2-6-8(3-5)18-10(12-6)9-13-7(4-17-9)11(15)16/h1-3,7,14H,4H2,(H,15,16)/t7-/m1/s1

Clé InChI

BJGNCJDXODQBOB-SSDOTTSWSA-N

Vous recherchez des produits similaires ? Visite Guide de comparaison des produits

Description générale

D-Luciferin is a small molecule present abundantly in bioluminescent organisms. This molecule is sensitive to oxygen and light.

Application

D-Luciferin has been used in determining luciferase activity by luciferase assay in mice and Arabidopsis thaliana seeds. It has also been used in measuring bioluminescence in neonatal rat ventricular cardiomyocytes.
Substrate for firefly luciferase with a Km of approx 2 μM.

Actions biochimiques/physiologiques

D-Luciferin uptake within the cells is regulated by the adenosine triphosphate (ATP)-binding cassette (ABC) transporter inhibitors like ATP-binding cassette transporter G2 (ABCG2)/ breast cancer resistance protein (BCRP) inhibitor fumitremorgin C. This substrate is oxidized by firefly luciferase that results in emitting a photon, which has the capability of passing through living tissues.

Application

Réf. du produit
Description
Tarif

Remplacé(e)(s) par

Réf. du produit
Description
Tarif

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Faites votre choix parmi les versions les plus récentes :

Certificats d'analyse (COA)

Lot/Batch Number
SLCD6367
SLBS2450V
SLBN3353V
SLBG0886V
030M5001V

Vous ne trouvez pas la bonne version ?

Si vous avez besoin d'une version particulière, vous pouvez rechercher un certificat spécifique par le numéro de lot.

Rechercher un(e) COA

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Les clients ont également consulté

Slide 1 of 1

1 of 1

Firefly Luciferase HTS Assay Glow-type luciferase assay for the quantification of firefly luciferase gene expression in mammalian cells with signal half-life of about 3 hours

Sigma-Aldrich

SCT150

Firefly Luciferase HTS Assay

Imaging and Spectroscopic Analysis of Living Cells
Test (2012)
Jamie F S Mann et al.
Journal of virology, 88(12), 6959-6969 (2014-04-11)
It is accepted that an effective prophylactic HIV-1 vaccine is likely to have the greatest impact on viral transmission rates. As previous reports have implicated DNA-priming, protein boost regimens to be efficient activators of humoral responses, we sought to optimize
Caglar Cekic et al.
Cancer research, 74(24), 7239-7249 (2014-10-25)
Adenosine A(2A) receptor (A(2A)R) blockade enhances innate and adaptive immune responses. However, mouse genetic studies have shown that A(2A)R deletion does not inhibit the growth of all tumor types. In the current study, we showed that growth rates for ectopic
Floris P R Verbeek et al.
Annals of surgical oncology, 21 Suppl 4, S528-S537 (2014-02-12)
Irradical tumor resections and iatrogenic ureteral injury remain a significant problem during lower abdominal surgery. The aim of the current study was to intraoperatively identify both colorectal tumors and ureters in subcutaneous and orthotopic animal models using cRGD-ZW800-1 and near-infrared
Erik Wennerberg et al.
Cancer immunology, immunotherapy : CII, 64(2), 225-235 (2014-10-27)
Adoptive infusion of natural killer (NK) cells is being increasingly explored as a therapy in patients with cancer, although clinical responses are thus far limited to patients with hematological malignancies. Inadequate homing of infused NK cells to the tumor site
Afficher tous les articles scientifiques apparentés

Articles

TokeOni: Properties and Applications

Bioluminescence imaging (BLI) systems allows for high-sensitive and noninvasive monitoring of cell proliferation, activity of signaling pathways and protein-protein interactions in living tissues.

TokeOni Analog - seMpai: Properties and Applications

Overall results suggest that seMpai is a suitable substrate of near-infrared BLI for many biological experiments. Its high solubility in neutral buffer conditions further extends the bioluminescent application of TokeOni derivatives.

Bioluminescent Firefly Luciferase Assays

Low cost and high performing bioluminescent Firefly Luciferase and Renilla Luciferase assays for gene reporter and ATP cell viability experiments. Benefits of dual luciferase assays also discussed.

Chromatograms

application for HPLCapplication for HPLC

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique