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L4544

Sigma-Aldrich

Human Laminin

from human fibroblasts, cell culture derived, liquid, 0.5 mg/mL, suitable for cell culture

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About This Item

Numéro MDL:
Code UNSPSC :
12352202
Nomenclature NACRES :
NA.75

product name

Laminin from human fibroblasts, cell culture derived, liquid, sterile-filtered

Source biologique

human fibroblasts

Niveau de qualité

Stérilité

sterile-filtered

Forme

liquid

Conditionnement

pkg of 100 μL

Technique(s)

cell culture | mammalian: suitable

Couverture de surface

1‑2 μg/cm2

Numéro d'accès UniProt

Spécificité de la liaison

Peptide Source: Collagen

Conditions d'expédition

dry ice

Température de stockage

−70°C

Informations sur le gène

human ... LAMB1(3912)

Application

Laminin from human fibroblasts has been used:
  • in coating six-well plates for wound healing assay
  • as a substrate in cell adhesion and spreading assay
  • for differentiation of human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs) toward dopaminergic neurons
  • in corneal endothelial cell wound healing (migration) assay and corneal endothelial cell barrier assay

Laminin from human fibroblasts is recommended for use as a cell culture substratum at 1-2 μg/cm2. The optimal concentration does depend on cell type as well as the application or research objectives.

Actions biochimiques/physiologiques

Laminin proteins are integral components of structural scaffolding in animal tissues. They associate with type IV collagen via entactin and perlecan and bind to cell membranes through integrin receptors, dystroglycan glycoprotein complexes and Lutheran blood group glycoproteins. Laminin has active domains for collagen binding, cell adhesion, heparin binding, and neurite outgrowth fragment. Laminin supports growth and differentiation of many cell types including epithelial, endothelial, neural, muscle and liver cells.

Composants

Laminin is an extracellular matrix multidomain trimeric glycoprotein, and is the main non-collagenous component of basal lamina that supports adhesion, proliferation and differentiation. Laminin is composed of both A, B1 and B2 chains, which are connected by many disulfide bonds. This laminin product is produced by human fibroblasts and epithelial cells in a co-culture system and then purified biochemically.

Attention

It is recommended to store this product at -70°C, where it will remain stable for two years.

Notes préparatoires

This product is supplied at a concentration of 0.5 mg/mL in TBS. Thaw this solution slowly before use at 2-8°C to avoid gel formation. If at any time prior to that, the laminin is allowed to warm up prematurely, gelling can occur. Moreover, even allowing it to be stored at -20C can induce gelling or significantly reduce its activity or both." For use as a coating, dilute in a HBSS, coat culture surface with a minimal volume and incubate at 37°C for 1-2 hours. Wash 3 times with HBBS before plating cells. Laminin coatings can be stored for one month at 2-8°C.

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

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Joseph JV, et al
PLoS ONE, 10(12), e0145393-e0145393 (2015)
Highly efficient neural conversion of human pluripotent stem cells in adherent and animal-free conditions
Lukovic D, et al.
Stem Cells Translational Medicine, 6(4), 1217-1226 (2017)
PRL-3 engages the focal adhesion pathway in triple-negative breast cancer cells to alter actin structure and substrate adhesion properties critical for cell migration and invasion
Gari HH, et al.
Cancer letters, 380(2), 505-512 (2016)
Danielle K Lewis et al.
Aging cell, 7(6), 836-849 (2008-09-10)
Astrocytes comprise a large proportion of the central nervous system support cells and play a critical role in neural injury and repair. The present study examined the impact of ovarian aging using an ex vivo model system, where astrocytes were

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