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K3753

Sigma-Aldrich

Krebs-Henseleit Buffer Modified

With 2000 mg/L glucose, without calcium chloride and sodium bicarbonate, powder, suitable for cell culture

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About This Item

Code UNSPSC :
12352207

Niveau de qualité

Forme

powder

Technique(s)

cell culture | mammalian: suitable

Température de stockage

2-8°C

Description générale

Krebs-Henseleit Buffer Modified is a balanced salt solution (BSS) that is used in cell culture and plays a role in providing cells with water and inorganic salts for normal cell metabolism. In addition, it provides a buffering system to maintain the optimal pH of the medium and acts as an irrigating, transporting, and diluting agent to maintain the intra- and extra-cellular osmotic balance. BSS provides glucose as an energy source for cell metabolism.

Application

Krebs-Henseleit Buffer Modified has been used:

  • to collect the rat brains for ex vivo brain slice experiments
  • to measure calcium and sodium in rat pulmonary arterial endothelial cells (PAECs)
  • to collect the uterine and forelimb blood vessel samples from horses

Quantité

Formulated to contain 9.6 grams of powder per liter of medium.

Reconstitution

Supplement with 2.1 g/L sodium bicarbonate.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


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Yermek Nigmet et al.
Transplantation, 103(3), 512-521 (2018-11-22)
Current critical shortage of donor organs has increased the use of donation after circulatory death (DCD) livers for transplantation, despite higher risk for primary nonfunction or ischemic cholangiopathy. Human atrial natriuretic peptide (hANP) is a cardiovascular hormone that possesses protective
Shulin Li et al.
Stem cell research & therapy, 12(1), 27-27 (2021-01-09)
The therapeutic effect of mesenchymal stem cells (MSCs) from human adipose tissue on renal interstitial fibrosis has been demonstrated by several groups. However, the way to enhance the renoprotective effect of adipose-derived mesenchymal stem cells (AMSCs) and the possible mechanisms
Ignasi Jorba et al.
Journal of the mechanical behavior of biomedical materials, 71, 106-113 (2017-03-13)
Recent evidence suggests that obstructive sleep apnea (OSA) may increase the risk of Alzheimer´s disease (AD), with the latter promoting alterations in brain tissue stiffness, a feature of ageing. Here, we assessed the effects of age and intermittent hypoxia (IH)
Xiaoqian Zhang et al.
International journal of molecular medicine, 39(1), 101-112 (2016-12-14)
Cell encapsulation provides a three-dimensional support by incorporating isolated cells into microcapsules with the goal of simultaneously maintaining cell survival and function, as well as providing active transport for a bioreactor in vitro similarly to that observed in vivo. However, the biotra-nsformation
J L Klotz et al.
Journal of animal science, 95(11), 5151-5160 (2018-01-03)
Mares grazing endophyte-infected () tall fescue () typically exhibit reproductive dysfunction rather than problems associated with peripheral vasoconstriction as a primary sign of the fescue toxicosis syndrome. Research using Doppler ultrasonography demonstrated that consumption of endophyte-infected tall fescue seed causes

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