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J4750

Sigma-Aldrich

Anti-JNK, Activated (Diphosphorylated JNK) antibody, Mouse monoclonal

clone JNK-PT48, purified from hybridoma cell culture

Synonyme(s) :

Monoclonal Anti-JNK, Activated (Diphosphorylated JNK) antibody produced in mouse

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.44

Source biologique

mouse

Conjugué

unconjugated

Forme d'anticorps

purified from hybridoma cell culture

Type de produit anticorps

primary antibodies

Clone

JNK-PT48, monoclonal

Forme

buffered aqueous solution

Poids mol.

antigen 46 kDa
antigen 54 kDa

Espèces réactives

mouse, human, rat

Concentration

~2 mg/mL

Technique(s)

immunocytochemistry: suitable
indirect ELISA: suitable
microarray: suitable
western blot: 4-20 μg/mL using extract of human Jurkat cells activated with anisomycin

Isotype

IgG1

Numéro d'accès UniProt

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... MAPK8(5599)
mouse ... Mapk8(26419)
rat ... Mapk8(116554)

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Description générale

Monoclonal Anti-JNK, Activated (Diphosphorylated JNK) (mouse IgG1 isotype) is derived from the JNK-PT48 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to the phosphorylated form of JNK activation loop, conjugated to KLH. JNK (c-Jun N terminal protein kinase) belongs to the mitogen-activated protein kinase (MAPK) superfamily of enzymes. The kinases in the MAPK level are activated by phosphorylation of both tyrosine (Y) and threonine (T) residues organized in a TXY motif. The residue in between the two phosphorylated residue determines the specificity of activation of the MAPKs and is proline for JNK. JNK1-3 of molecular weights 46, 54, and 52 kDa respectively.

Spécificité

The antibody reacts specifically with the activated diphosphorylated form of JNK. The epitope recognized by the antibody contains the regulatory site of JNK (Thr183 and Tyr185). Weak cross-reaction is observed with the monophosphorylated threonine peptide, but not with the non-phosphorylated or monophosphorylated tyrosine peptides of JNK. It does not recognize any form of p38-MAPK or ERK-MAPK.

Immunogène

synthetic peptide sequence containing amino acids pTPpYVVTRYYR, corresponding to the phosphorylated form of JNK-activation loop.

Application

Monoclonal Anti-JNK, Activated (Diphosphorylated JNK) antibody has been used in immunoblotting, enzyme linked immunosorbent assay (ELISA), immunocytochemistry, western blotting, immunohistochemistry.

Actions biochimiques/physiologiques

c-Jun N terminal protein kinase (JNK) is mostly stress-responsive kinase and have been implicated in cell death in several cellular systems. The JNK cascade is triggered by small GTPases that are Rac and Cell division control protein 42 (CDC42). After activation, JNKs translocate to the nucleus where they physically associate with and activate their target transcription factors (cJun, AMP-dependent transcription factor (ATF) etc.).

Forme physique

Solution in phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

nwg

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

JNK signaling: regulation and functions based on complex protein-protein partnerships
Zeke A, et al.
Microbiology and Molecular Biology Reviews, 80(3), 793-835 (2016)
Activation and cellular localization of the p38 and JNK MAPK pathways in rat crescentic glomerulonephritis
Stambe C, et al.
Kidney International, 64(6), 2121-2132 (2003)
JNK-signaling: a multiplexing hub in programmed cell death
Dhanasekaran, Danny N and Reddy, E Premkumar
Genes & cancer, 8(9-10), 682-682 (2017)
Pigment Epithelium-Derived Factor Alleviates Tamoxifen-Induced Endometrial Hyperplasia
Goldberg K, et al.
Molecular Cancer Therapeutics, 14(12), 2840-2849 (2015)
Cosimo Stambe et al.
Kidney international, 64(6), 2121-2132 (2003-11-25)
The p38 and c-Jun N-terminal kinase (JNK) mitogen-activated protein kinase (MAPK) are intracellular signal transduction pathways involved in the production of inflammatory mediators. Little, however, is known about the contribution of these pathways to renal inflammation, nor the cell types

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