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I9889

Sigma-Aldrich

Anti-Human IgA (α-chain specific) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

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About This Item

Numéro MDL:
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.46

Source biologique

rabbit

Conjugué

unconjugated

Forme d'anticorps

IgG fraction of antiserum

Type de produit anticorps

secondary antibodies

Clone

polyclonal

Forme

buffered aqueous solution

Technique(s)

: 2.5-5.0 mg/mL using Antibody concentration by precipitin Vs Human serum
: 4.0-6.0 mg/mL using Antibody concentration by precipitin Vs Human whey
Ouchterlony double diffusion: 1:32

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

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Description générale

IgA is the highest generated antibody isotype. IgAs are mainly secreted in the intestinal mucosa.
Anti-Human IgA (α-chain specific) antibody is specific for the α-chain of IgA in humans. The product reacts with human IgA when tested against purified human IgA, IgG, IgM, and Bence Jones κ and lambda myeloma proteins.
Immunoglobulin A (IgA) exists in two isotypic forms: IgA1 and IgA2. It is produced as secretory IgA (sIgA) and serum IgA. sIgA exists in a monomeric form with two heavy and two light chains. Serum IgA exists in the dimeric form linked by disulfide bind with an additional J chain. IgA is found in saliva, serum, colostrum and nasal fluid.

Spécificité

The antibody preparation is specific for human IgA when tested against purified human IgA, IgG, IgM, Bence Jones kappa and lambda myeloma proteins.

Immunogène

IgA purified from normal human colostrum

Application

Anti-Human IgA (α-chain specific) antibody is suitable for use in:
  • enzyme-linked immunosorbent assay (ELISA)
  • quantitative precipitin assay (2.5-5 mg/mL)
  • immunoelectrophoresis
  • Ouchterlony double diffusion (1:16)

Actions biochimiques/physiologiques

IgA is a secretory glycoprotein antibody that regulates the physiological balance between commensal intestinal bacteria and the defenses of the host immune system. It prevents the commensal microorganisms from invading the intestines.
Immunoglobulin A (IgA) mediates antibody-dependent cell-mediated cytotoxicity (ADCC) by binding to leukocytes and enhances microbial destruction. It acts as the first line of defense in antigen clearance. IgA initiates the alternative pathway of the complement system to perform protective functions. It reduces inflammation stimulated by the classical pathway complement activation. IgA prevents proteolysis and inhibits the uptake of bacterial, viral or antigen attachment.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide as preservative

Stockage et stabilité

For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

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Li CY, et al.
The Chinese Journal of Physiology, 58(4), 263-269 (2015)
N Rajan et al.
Infection and immunity, 67(10), 5027-5032 (1999-09-25)
Adherence of type 1-piliated Escherichia coli to carbohydrate structures of vaginal mucosa plays a major role in the pathogenesis of ascending urinary tract infections in women. Colonization of the vaginal introitus is influenced by interactions between pathogens, vaginal fluid, and
Handbook of Human Immunology (2008)
J Zakrzewski et al.
Infection, 26(2), 116-119 (1998-04-30)
Thirty-eight clinical isolates of Haemophilus influenzae and ten clinical isolates of Streptococcus pneumoniae were examined for IgA1 protease production. A suspension of surface material of each individual strain was incubated with human secretory IgA; IgA1 cleavage products were detected by
Salivary IgA from the sublingual compartment as
a novel noninvasive proxy for intestinal immune
induction
Aase A, et al.
Mucosal Immunology, 9(4), 884-884 (2016)

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