HPA005487

Anti-TPX2 antibody produced in rabbit

Prestige Antibodies^® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution

• Synonyme(s) : Anti-DIL-2, Anti-Differentially expressed in cancerous and non-cancerous lung cells 2, Anti-Hepatocellular carcinoma-associated antigen 519, Anti-Protein fls353, Anti-Restricted expression proliferation-associated protein 100, Anti-Targeting protein for Xklp2, Anti-p100
• Code UNSPSC : 12352203
• Numéro HPA (Human Protein Atlas): HPA005487
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Conjugué: unconjugated
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Gamme de produits: Prestige Antibodies^® Powered by Atlas Antibodies
• Forme: buffered aqueous glycerol solution
• Espèces réactives: human
• Validation améliorée: recombinant expression; orthogonal RNAseq; Learn more about Antibody Enhanced Validation
• Technique(s): immunoblotting: 0.04-0.4 μg/mL; immunofluorescence: 0.25-2 μg/mL; immunohistochemistry: 1:50-1:200
• Séquence immunogène: QELEKSMKMQQEVVEMRKKNEEFKKLALAGIGQPVKKSVSQVTKSVDFHFRTDERIKQHPKNQEEYKEVNFTSELRKHPSSPARVTKGCTIVKPFNLSQGKKRTFDETVSTYVPLAQQVEDFHKRTPNRYHLRSKKD
• Numéro d'accès UniProt: Q9ULW0
• Application(s): research pathology
• Conditions d'expédition: wet ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... TPX2(22974)

Description

Description générale

TPX2 (targeting protein for Xenopus kinesin-like protein 2) is a spindle assembly factor. It is a kinesin-like motor protein, which is directed toward the plus-end of microtubule. It localizes mainly to nucleus during interphase and has the highest level of expression during mitosis. This gene is localized to the human chromosomal locus 20q11.1. TPX2 protein has a molecular weight of 100kDa.

Immunogène

Targeting protein for Xklp2 recombinant protein epitope signature tag (PrEST)

Application

All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project and as a result, are supported by the most extensive characterization in the industry.

The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. These images and the collection of this vast data set can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. We also provide Prestige Antibodies^® protocols and other useful information.

Actions biochimiques/physiologiques

TPX2 (targeting protein for Xenopus kinesin-like protein 2) activates Aurora A and recruits it to spindles, and aids in spindle formation. This is achieved by the release of TPX2, during early mitosis, in a Ran-GTP dependent fashion. In mammals, it regulates the assembly of microtubules at kinetochores. This in turn is accomplished by the binding of microtubules to the COOH-terminal domain of Xenopus kinesin-like protein 2 (Xklp2). The expression of TPX2 is tightly regulated during different stages of cell cycle. Post cytokinesis, TPX2 levels decline rapidly, and remain as such through G1 phase. The expression levels increase again during S and G2 phase. This protein is also responsible for amplifying the response to DNA damage. TPX2 is implicated in the modulation of tumorigenesis in cervical cancer. It also plays a role in tumor growth and metastasis of human colon cancer.

Caractéristiques et avantages

Prestige Antibodies^® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies^® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section.

Every Prestige Antibody is tested in the following ways:

• IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues.
• Protein array of 364 human recombinant protein fragments.

Liaison

Corresponding Antigen APREST86576

Forme physique

Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide

Informations légales

Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)