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H9289

Sigma-Aldrich

Anti-Histone H3 (N-terminal) antibody produced in mouse

enhanced validation

affinity isolated antibody, buffered aqueous solution

Synonyme(s) :

Histone H3 Detection, Histone H3 Detection Antibody, Rabbit Anti-Histone H3

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

rabbit

Niveau de qualité

200

Conjugué

unconjugated

Forme d'anticorps

affinity isolated antibody

Type de produit anticorps

primary antibodies

Clone

polyclonal

Forme

buffered aqueous solution

Poids mol.

antigen ~17 kDa

Espèces réactives

human

Validation améliorée

independent
Learn more about Antibody Enhanced Validation

Technique(s)

indirect immunofluorescence: 2-4 μg/mL using A431 cells.
western blot: 0.2-0.5 μg/mL using PC12 cell lysate and A431 cell lysate.

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... HIST1H3A(8350)

Catégories apparentées

Description générale

Anti-Histone H3 (N-terminal) specifically recognizes human histone H3 (~17kDa). Histone H3/ histone cluster 1 H3 family member a (HIST1H3A) is a core member of the histone family. HIST1H3A is encoded by the gene mapped to human chromosome 6p22.2. The encoded protein is composed of ~136 amino acid.

Immunogène

synthetic peptide corresponding to amino acids 1-20 of human histone H3, conjugated to KLH.

Application

Anti-histone H3 (N-terminal) antibody produced in rabbit has been used in western blotting and native chromatin immunoprecipitation assay.

Actions biochimiques/physiologiques

Histones acts as structural scaffolds for DNA packaging. Histone H3 plays an important role in replication and DNA repair. Histone H3 is heavily post-translationally modified than the other members of histone family. Mutations in the gene leads to cerebellar high-grade gliomas.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Stockage et stabilité

For continuous use, store at 2-8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frostfree” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Autres remarques

In order to obtain the best results using various techniques and preparations, we recommend determining the optimal working dilutions by titration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Histone H3 K27M mutations in adult cerebellar high-grade gliomas.
Nakata S, et al.
Brain Tumor Pathology, 34, 113-119 (2017)
Hua Jiang et al.
Genes & development, 31(12), 1272-1287 (2017-07-27)
Hybrid seed lethality as a consequence of interspecies or interploidy hybridizations is a major mechanism of reproductive isolation in plants. This mechanism is manifested in the endosperm, a dosage-sensitive tissue supporting embryo growth. Deregulated expression of imprinted genes such as
Kerstin Müller et al.
PloS one, 7(12), e51532-e51532 (2012-12-15)
Plants have a remarkable ability to react to seasonal changes by synchronizing life-cycle transitions with environmental conditions. We addressed the question of how transcriptional re-programming occurs in response to an environmental cue that triggers the major life cycle transition from
Phosphorylation of histone H3 Ser10 establishes a hierarchy
for subsequent intramolecular modification events
Liokatis S, et al.
Nature Structural and Molecular Biology, 19, 819-823 (2012)
H3K9me3-binding proteins are dispensable for SETDB1/H3K9me3-dependent retroviral silencing
Maksakova IA, et al.
Epigenetics & Chromatin, 4 (2011)
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