H2662

Anti-Histone Deacetylase 7 (HDAC7) (KG-17) antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

• Synonyme(s) : Anti-HD7, Anti-HD7A, Anti-HDAC7A
• Numéro MDL: MFCD04040364
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Conjugué: unconjugated
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Forme: buffered aqueous solution
• Poids mol.: antigen ~105 kDa
• Espèces réactives: mouse, human, rat
• Technique(s): immunoprecipitation (IP): 1.0-1.5 μg using RIPA extract of 293T cells expressing recombinant human HDAC7; indirect immunofluorescence: 1:50 using rat NRK cells; western blot: 1:1,000 using whole extracts of mouse NIH-3T3 cells
• Numéro d'accès UniProt: Q8WUI4
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... HDAC7(51564) ; mouse ... Hdac7(56233) ; rat ... Hdac7a(84582)

Description

Description générale

Mammalian histone deacetylases (HDACs) can be divided into three classes according to sequence homology. Class I consists of the yeast transcriptional regulatory protein Rpd3-like proteins HDAC1, HDAC2, HDAC3, and HDAC8. Class II consists of the yeast Hda1-like proteins HDAC4, HDAC5, HDAC6, HDAC7, HDAC9 and HDAC10. Class III consists of the yeast sirtuin 2 (Sir2)-like proteins. Although HDAC7 is localized mostly to the cell nucleus, it is also found in the cytoplasm.

Spécificité

Anti-Histone Deacetylase 7 recognizes human, mouse, and rat HDAC7.

Immunogène

Synthetic peptide corresponding to amino acids of human HDAC7, conjugated to KLH.

Application

Anti-Histone Deacetylase 7 (HDAC7) (KG-17) antibody produced in rabbit has been used in

• indirect immunofluorescene
• immunoblotting
• immunoprecipitation

Actions biochimiques/physiologiques

Class II histone deacetylases (HDACs) have been implicated in the regulation of muscle differentiation. Interaction of HDAC4, -5, and -7 with members of the myocyte enhancer factor-2 (Mef2) family of transcription factors represses their transcriptional activity and prevents myogenesis. Shuttling of HDAC7 between the cell nucleus and the cytoplasm is controlled by a mechanism involving calmodulin independent kinase I (CaMKI) and 14-3-3 proteins. The HDAC7 enzymatic activity depends on its interaction with the class I HDAC3, and the corepressors silencing mediator of retinoic acid and thyroid hormone receptors (SMRT) and nuclear receptor co-repressor (N-COR). HDAC7 also interacts with the transcriptional repressor B-cell lymphoma 6 (BCL-6).

Forme physique

Solution in 0.01 M phosphate buffered saline containing 1% bovine serum albumin and 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): nwg
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable