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Key Documents

GE28-9889-53

Sigma-Aldrich

XK 50/30 Column

Synonyme(s) :

XK 50/30 Column

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About This Item

Code UNSPSC :
41115711
Nomenclature NACRES :
SB.52

Technique(s)

LPLC: suitable

Description générale

XK empty columns are designed for liquid chromatography at low to medium pressure. The user-friendly design ensures trouble-free operation with fittings for direct connection to ÄKTA design instruments or other high-performance liquid chromatography systems. The product family is based on a robust design for high reproducibility and accurate results. XK columns are compatible with aqueous solutions and most organic solvents used in liquid chromatography of macromolecules.
XK empty columns provide reliable and reproducible separations. The tube material is of borosilicate glass, which allows for visual inspection of the media bed. The columns are made of biocompatible materials that exhibit excellent chemical resistance. A broad range of column dimensions makes XK columns suitable for many different purification needs. Column packing can be performed using either a packing reservoir or an extra column tube attached with a packing connector. The QuickLock mechanism of the adapter shaft facilitates rapid and easy movement of the adapter, simplifying adjustments of the bed height, disassembly, and cleaning. The design of the adapter plunger gives a uniform flow, which maintains the integrity of the packed bed during operations. An extensive range of spare parts is available, facilitating maintenance.

Caractéristiques et avantages

Adapter QuickLock mechanism that facilitates column handling and cleaning
Cooling jacket for sensitive purifications
Fittings for direct connection to chromatographiy systems
Plunger design to ensure a homogeneous buffer distribution and reproducible packing
User friendly and robust empty columns, designed for liquid chromatography at low to medium pressure.

Certificats d'analyse (COA)

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Articles

This page describes efficient column packing and preparation for affinity chromatography of antibodies.

This page shows how to perform sample desalting and buffer exchange for affinity chromatography of antibodies.

Protocoles

This page covers how and why to use Sepharose Big Beads for purification from crude, viscous samples.

This page shows how to purify histidine-tagged proteins using TALON Superflow from GE Healthcare.

This page shows how to separate IgG antibodies by affinity chromatography using Protein G Sepharose 4 Fast Flow from GE Healthcare.

This page shows how to separate IgG antibodies by affinity chromatography using Protein G Sepharose 4 Fast Flow from GE Healthcare.

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