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Key Documents

GE28-4064-16

Sigma-Aldrich

Tricorn 10/150 Column

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About This Item

Code UNSPSC :
12352200
Nomenclature NACRES :
SB.52

Technique(s)

LPLC: suitable

Catégories apparentées

Description générale

Tricorn 5 and 10 Coarse Filter Kit is used for large volume/repeated loading of clarified sample to reduce the risk of clogging in combination with capture media. Both filter and coarse filter kits include top and bottom adapters, bottom filters, and EPDM O-rings, five of each.
Tricorn Packing Equipment includes the Packing Equipment 5/50 (5-mm connector and 50-mm glass tube) or 10/100 (10-mm connector and 100-mm glass tube), EPDM O-rings and bottom unit, cap, and stop plug.

Caractéristiques et avantages

Broad range of column dimensions suitable for many different purification needs.
Glass tubes coated with plastic film prevent harm if the column is dropped or operated outside its specification.
Locking ring prevents accidental compression of media bed.
Separate packing connector allows simple column packing for a broad range of media.
The modern integrated, snap-on design results in a column that is easy to assembly, operate, and maintain.
Tricorn empty columns are designed for high performance separations on small and medium scale. Pressure stability up to 100 bar and durable column material ensure reliable and reproducible operation as well as compatibility with the majority of the commercial lab media.

Certificats d'analyse (COA)

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Articles

This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.

This page covers detailed aspects of each step in an IEX separation to improve resolution and overall performance.

Protocoles

Here we discuss purification options when working with Sepharose High Performance and we present some examples of media screening, capture and purification.

This page covers the use of Sepharose High Performance media for purification of proteins, peptides or oligonucleotides, when to use them, and with which systems.

This page discusses how to do a purification with SOURCE™ media from GE Healthcare

Column Packing and Preparation for Size Exclusion Chromatography

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