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GE17-5478-02

Capto™ L

Cytiva 17-5478-02, pack of 200 mL

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About This Item

Code UNSPSC :
41106500
Nomenclature NACRES :
NA.56

ligand

recombinant protein L (E. coli), mammalian free

Conditionnement

pack of 200 mL

Fabricant/nom de marque

Cytiva 17-5478-02

Conditions de stockage

(20% Ehtanol)

Matrice

rigid, highly cross-linked agarose

Taille moy. des particules

85 μm (d₅₀v is the median particle size of the cumulative volume distribution)

working range

2-10(pH interval where the medium can be operated without significant change in function.)

Adéquation

suitable for bioprocess medium

Température de stockage

2-8°C

Catégories apparentées

Description générale

Capto™ L resin is designed for antibody fragment purification, when the fragment contains the kappa light chain.

Affinity resin for antibody fragment purification

Capto™ L combines a rigid, high-flow agarose matrix with the immunoglobulin-binding recombinant protein L ligand, which has a strong affinity to the variable region of antibody kappa light chains. The high binding capacity and affinity to a broad range of antibody fragments makes this resin an excellent capture platform for antibody fragments containing kappa light chains. Common uses include domain antibody (dAb), scFv, and Fab purification.

Using this protein L resin

Capto™ L resin requires minimal optimization and produces high purity in one separation step, resulting in significant savings during process development. This resin is also advantageous in manufacturing, because high selectivity of affinity capture reduces the number of subsequent polishing steps.

Application

Purification of antibody fragments using affinity chromatography

Caractéristiques et avantages

  • Suitable for the capture of a wide range of antibody fragments such as Fabs, dAbs, and single-chain fragment variable (scFv) .
  • High binding capacity, low ligand leakage, and selectivity for a broad range of antibody fragments reduces process time and amount of resin.
  • Enables a platform approach to antibody fragment purification.Excellent productivity, process economy, and process design flexibility due to rigid matrix.
  • Hydrophilic base matrix ensures low levels of non-specific binding and low levels of host cell-derived impurities in the elution pool.
  • Fulfills industrial demands for security of supply, robust performance, and regulatory support.

Stockage et stabilité

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Remarque sur l'analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Informations légales

Capto is a trademark of Cytiva

Pictogrammes

Flame
GHS02

Mention d'avertissement

Warning

Mentions de danger

H226

Code de la classe de stockage

3 - Flammable liquids

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Protocoles

Performing a Purification of Antibody Fragments with Capto L Products

This page shows how to purify antibody fragments by affinity chromatography using Capto L products from GE Healthcare.

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