GE17-5080-01
HiTrap® rprotein A Fast Flow
Cytiva 17-5080-01, pack of 1 × 5 mL
About This Item
Produits recommandés
ligand
recombinant protein A (E. coli)
Durée de conservation
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Conditionnement
pack of 1 × 5 mL
Fabricant/nom de marque
Cytiva 17-5080-01
Conditions de stockage
(20% Ehtanol)
(20% Ethanol)
Paramètres
<20 mL/min flow rate (The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.)
42 psi
Taille du lit
16 mm × 25 mm
Volume du lit
5 mL
D.I. de la colonne
16 mm
Matrice
4% cross-linked agarose
Taille des particules
60-165 μm
Diamètre moyen
90 μm (d50v)
cleaning
2-11(pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.)
working range
3-10
Capacité
~50 mg binding capacity (human lgG/ml medium)(Capacity of HiTrap rProtein A for some monoclonal antibodies. Running conditions: Binding buffer: 20 mM sodium phosphate (incl. 3 M NaCl for IgG), pH 7.0. Elution buffer: 0.1 M sodium citrate, pH 3.0.)
Adéquation
suitable for bioprocess medium
Température de stockage
2-8°C
Catégories apparentées
Description générale
The recombinant protein A ligand (produced in E. coli) is coupled to Sepharose™ Fast Flow by a technique which generates a stable thioether linkage between rProtein A and the base matrix. The coupling technique is optimized to give high binding capacity for IgG.
Caractéristiques et avantages
- Quick, convenient preparative purification of monoclonal antibodies.
- Well-established protein A-based medium used in many approved MAb processes.
- Recombinant protein A exhibits similar Fc region specificity to that of native protein A but shows enhanced binding capacity and fewer regulatory concerns due to the total absence of mammalian culture in the ligand production and purification.
- Simple operations with a syringe, pump, or high-performance chromatography system, such as AKTA design.
Remarque sur l'analyse
Informations légales
Mention d'avertissement
Warning
Mentions de danger
Conseils de prudence
Code de la classe de stockage
3 - Flammable liquids
Certificats d'analyse (COA)
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Articles
This page shows how to separate IgG antibodies by affinity chromatography using HiTrap® Protein G HP columns from GE Healthcare.
This page shows various purification options for Protein A Sepharose chromatography media and describes typical binding and elution conditions for Protein A Sepharose chromatography media.
This page shows how to solve practical problems that may occur when running an affinity chromatography column.
Protocoles
This page shows how to perform a separation with Protein A, an affinity chromatography binder used in HiTrap Protein A HP, Protein A Sepharose 4 Fast Flow, HiTrap rProtein A FF, rProtein A Sepharose 4 Fast Flow and MabSelect products from GE Healthcare.
This page shows how to convert between linear flow and volumetric flow rates in affinity chromatography.
This page shows how to perform column packing and preparation for affinity chromatography when using Tricorn™ or XK columns available from GE Healthcare.
This page shows how to prepare samples for purification with affinity chromatography.
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