FG0465

Iscove′s Modified Dulbecco′s Medium

With L-alanyl-L-glutamine and sodium bicarbonate, sterile-filtered, liquid, suitable for cell culture

• Code UNSPSC : 12352207

Propriétés

• Niveau de qualité: 400
• Stérilité: sterile-filtered; sterile
• Gamme de produits: AQmedia^™
• Forme: liquid
• Technique(s): cell culture | mammalian: suitable
• Impuretés: Endotoxin, tested
• Composants: stable glutamine: yes; NaHCO₃: yes; glucose: yes; phenol red: yes
• Conditions d'expédition: ambient
• Température de stockage: 2-8°C

Description

Description générale

This IMDM differs from the original formula, because it is supplemented with L-alanyl-L-glutamine dipeptide. Glutamine is notoriously unstable in cell culture. This dipeptide provides a more stable form of glutamine for use in cell cultures.

Application

Guilbert and Iscove demonstrated that precursor cells of erythrocytes and macrophages could be cultured in a totally defined serum free medium supplemented with albumin, transferrin, lecithin, and selenium. This medium is a modification of Dulbecco¢s Modified Eagle¢s Medium (DME) containing selenium, additional amino acids and vitamins, sodium pyruvate, HEPES buffer, and potassium nitrate instead of ferric nitrate. Further studies demonstrated that Iscove¢s Medium would support murine B lymphocytes, hemopoietic tissue from bone marrow, B cells stimulated with lipopolysaccharide, T lymphocytes, and a variety of hybrid cells.

Informations légales

AQmedia is a trademark of Sigma-Aldrich Co. LLC

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable