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F4890

Sigma-Aldrich

Monoclonal Anti-Rabbit Immunoglobulins–FITC antibody produced in mouse

clone RG-16, purified immunoglobulin, buffered aqueous solution

Synonyme(s) :

Monoclonal Anti-Rabbit Immunoglobulins (IgG, IgA, IgM)

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.46

Source biologique

mouse

Conjugué

FITC conjugate

Forme d'anticorps

purified immunoglobulin

Type de produit anticorps

secondary antibodies

Clone

RG-16, monoclonal

Forme

buffered aqueous solution

Ne doit pas réagir avec

pig, chicken, bovine, horse, human, guinea pig, turkey, rat, canine, goat, feline, sheep

Conditions de stockage

protect from light

Technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:20
indirect immunofluorescence: 1:50 using using chicken fibroblast cells and primary antibody produced in rabbit

Isotype

IgG1

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

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Description générale

Reacts with an epitope on the heavy chain of rabbit IgG, IgA and IgM, which is sensitive to reduction. No cross-reactivity with human IgG, IgA and IgM.

Spécificité

Binds only rabbit Igs. Does not react with reduced rabbit Igs.

Application

Monoclonal Anti-Rabbit Immunoglobulins-FITC antibody produced in mouse has been used in:
  • immunocytochemistry
  • immunohistochemistry
  • immunofluorescence

The mouse monoclonal anti-Rabbit IgG (heavy chain-specific) peroxidase conjugated antibody can be used as a secondary antibody for western blot analysis of protein preps from E. coli cultures at a concentration of 1:10000 diluted in PBSt for 1 hour at room temperature.

Forme physique

Solution in 0.01 M sodium phosphate buffer, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

nwg

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Tarrah K Dilley et al.
Experimental cell research, 290(1), 38-48 (2003-10-01)
Aging is the highest risk factor for cancer. Although oxidants are thought to contribute to both aging and cancer, the interplay between oxidative stress, aging, and cancer has not been well studied. Human diploid fibroblasts (HDFs) undergo premature senescence in
F R Poulsen et al.
Experimental brain research, 135(3), 319-330 (2001-01-09)
Heparin affin regulatory peptide (HARP), also known as pleiotrophin or heparin-binding growth-associated molecule, is a developmentally regulated extracellular matrix protein that induces cell proliferation and promotes neurite outgrowth in vitro as well as pre- and postsynaptic developmental differentiation in vivo.
A study of the process of apoptosis in animals infected with the contagious ecthyma virus
Garrido-Farina GI, et al.
Veterinary Microbiology, 129(1-2), 28-39 (2008)
Increased synthesis of heparin affin regulatory peptide in the perforant path lesioned mouse hippocampal formation
Poulsen FR, et al.
Experimental Brain Research. Experimentelle Hirnforschung. Experimentation Cerebrale, 135(3), 319-330 (2000)
Inmaculada Couso et al.
Journal of experimental botany, 69(6), 1355-1367 (2017-10-21)
Autophagy is an intracellular catabolic process that allows cells to recycle unneeded or damaged material to maintain cellular homeostasis. This highly dynamic process is characterized by the formation of double-membrane vesicles called autophagosomes, which engulf and deliver the cargo to
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