ECM002
Thrombospondin-1 human
recombinant, expressed in HEK 293 cells, lyophilized powder, suitable for cell culture
Synonyme(s) :
THBS, THBS1, TSP, TSP1, Thrombospondin-1
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About This Item
Code UNSPSC :
12352202
Nomenclature NACRES :
NA.75
Produits recommandés
Source biologique
human
Niveau de qualité
Produit recombinant
expressed in HEK 293 cells
Stérilité
sterile
Pureté
≥95% (SDS-PAGE)
Forme
lyophilized powder
Poids mol.
127.5 kDa (The protein migrates as a 140 kDa band on SDS-PAGE due to glycosylation.)
Conditionnement
pkg of 50 μg
Technique(s)
cell culture | mammalian: suitable
Impuretés
≤1 EU/μg endotoxin, tested
Solubilité
water: soluble
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
dry ice
Température de stockage
−20°C
Informations sur le gène
human ... THBS1(7057)
Description générale
Thrombospondin-1 (TSP1) belongs to family of glycoproteins and possesses a complex trimeric structure. The structure comprises a heparin-binding domain and a procollagen homology domain located at the amino terminus, while the carboxyl-terminal end contains type I, II, and III repeats. This protein is manufactured in human cells using an all-human production system, with no serum. The human cells expression system allows human-like glycosylation and folding, and often supports better stability of the protein in culture. Recombinant human thrombospondin-1 is expressed in HEK293 cells as a glycoprotein with a calculated moleculaer mass of 127.5 kDa.
Application
Thrombospondin 1 human (TSP1) has been used:
- To treat hepatocyte (IHH) cancer cells to study the role of aspartyl protease 1 (MfSAP1) in extracellular matrix degradation.
- In in vitro Extracellular Matrix (ECM) protein degradation assays.
- In rat C6 astroglioma cells, for coating the cell culture plates to study the effects of integrins on CNTF (ciliary neurotrophic factor) expression.
- In the preparation of secreted factor cocktail for analysis of the human mesenchymal stem cell secretome.
Recommended for use as a cell culture substratum at 1-5 μg/cm2 or 0.1-25 μg/ml. Optimal concentration depends on cell type as well as the application or research objectives.
Actions biochimiques/physiologiques
Thrombospondin (TSP1) plays a crucial role in guiding extracellular matrix (ECM) synthesis and facilitating dynamic tissue remodeling. It is involved in various biological processes including angiogenesis, matrix assembly, activation of transforming growth factor (TGF), and promotion of neurite outgrowth. TSP1 binds to various cell surface receptors, such as integrins and integrin-associated protein CD47. Studies have also shown that the signaling pathway involving TSP1-CD47 promotes the generation of reactive oxygen species (ROS) in human pulmonary artery endothelial cells. This signaling pathway plays a significant role in the manifestation of sickle cell-associated vasculopathy and the subsequent progression of pulmonary hypertension. Moreover, hypoxia-inducible factor (HIF)-2α has been shown to upregulate the expression of TSP1 under hypoxic conditions. TSP1 also plays a crucial role in inflammatory processes and post-inflammatory tissue dynamics. It is upregulated in rheumatoid synovial tissues and might be associated with rheumatoid arthritis.
Caractéristiques et avantages
- Human-derived thrombospondin
- Produced through recombinant expression in HEK 293 cells
- Low endotoxin levels
Forme physique
Supplied as a powder, lyophilized from phosphate buffered saline.
Remarque sur l'analyse
The biological activity of recombinant human thrombospondin-1 was tested in culture by measuring the ability of immobilized DTT-treated thrombospondin-1 to support adhesion of SVEC4-10 cells.
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 2
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Gesine M Dittrich et al.
Basic research in cardiology, 116(1), 26-26 (2021-04-21)
Heart failure due to high blood pressure or ischemic injury remains a major problem for millions of patients worldwide. Despite enormous advances in deciphering the molecular mechanisms underlying heart failure progression, the cell-type specific adaptations and especially intercellular signaling remain
Si En Poh et al.
Frontiers in cellular and infection microbiology, 10, 148-148 (2020-04-25)
Malassezia is the most abundant eukaryotic microbial genus on human skin. Similar to many human-residing fungi, Malassezia has high metabolic potential and secretes a plethora of hydrolytic enzymes that can potentially modify and structure the external skin environment. Here we
Samira Ghorbani et al.
Nature communications, 13(1), 2445-2445 (2022-05-05)
Remyelination failure in multiple sclerosis (MS) contributes to progression of disability. The deficient repair results from neuroinflammation and deposition of inhibitors including chondroitin sulfate proteoglycans (CSPGs). Which CSPG member is repair-inhibitory or alters local inflammation to exacerbate injury is unknown.
Articles
Attachment Factors for 3-Dimensional Cell Culture
The extracellular matrix (ECM) is secreted by cells and surrounds them in tissues.
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
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