E9033
pFLAG-Myc-CMV™-22 Expression Vector
Shuttle vector for transient or stable intracellular expression of dual tagged N-terminal FLAG and C-terminal c-myc fusion proteins
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About This Item
Code UNSPSC :
12352200
Produits recommandés
Étiquette/Marqueur
FLAG® tagged
c-Myc tagged (C-terminal)
Qualité
for molecular biology
Forme
buffered aqueous solution
Conditions d'expédition
dry ice
Température de stockage
−20°C
Description générale
The pFLAG-Myc-CMV™-22 Expression Vector is a 6.3 kb derivative of pCMV5 used to establish transient or stable intracellular expression of dual-tagged N-terminal FLAG® and C-terminal c-myc fusion proteins in mammalian cells. The vector encodes the FLAG epitope (Asp-Tyr-Lys-Xaa-Xaa-Asp) and a c-myc epitope (EQKLISEEDL) upstream and downstream of the multiple cloning sites, respectively.
pFLAG-Myc-CMV-22 Expression Vector is a shuttle vector for E. coli and mammalian cells. Efficiency of replication is optimal when using an SV40 T antigen expressing host.
The pFLAG-CMV-4-BAP Control Plasmid is a 7.7 kb derivative of pCMV5 used for transient intracellular expression of N-terminal Met-FLAG bacterial alkaline phosphatase fusion protein in mammalian cells.
Vector Maps and Sequences
pFLAG-Myc-CMV-22 Expression Vector is a shuttle vector for E. coli and mammalian cells. Efficiency of replication is optimal when using an SV40 T antigen expressing host.
The pFLAG-CMV-4-BAP Control Plasmid is a 7.7 kb derivative of pCMV5 used for transient intracellular expression of N-terminal Met-FLAG bacterial alkaline phosphatase fusion protein in mammalian cells.
Vector Maps and Sequences
Application
The pFLAG-Myc-CMV™-20 Expression Vector is suitable for stable cytoplasmic expression of C-terminal c-myc N-terminal Met-FLAG® fusion proteins in mammalian cells.
Composants
pFLAG-Myc-CMV™-22 Expression Vector 20 μg (E5901) is supplied as 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA.
pFLAG-CMV™-4-BAP Control Plasmid 20 μg (C4722) is supplied as 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA.
pFLAG-CMV™-4-BAP Control Plasmid 20 μg (C4722) is supplied as 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA.
Principe
The promoter-regulatory region of the human cytomegalovirus drives transcription of FLAG® and c-myc fusion constructs. The aminoglycoside phosphotransferase II gene (Neo-r) confers resistance to aminoglycosides such as G418 allowing for selection of stable transfectants.
Informations légales
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
pFLAG-CMV is a trademark of Sigma-Aldrich Co. LLC
pFLAG-Myc-CMV is a trademark of Sigma-Aldrich Co. LLC
Produit(s) apparenté(s)
Réf. du produit
Description
Tarif
Code de la classe de stockage
10 - Combustible liquids
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