E0157
Anti-eIF2α antibody produced in rabbit
IgG fraction of antiserum, buffered aqueous solution
Synonyme(s) :
Anti-EIF2, Anti-EIF2S1, Anti-Eukaryotic translation initiation factor 2, Anti-eIF-2A
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About This Item
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
rabbit
Conjugué
unconjugated
Forme d'anticorps
IgG fraction of antiserum
Type de produit anticorps
primary antibodies
Clone
polyclonal
Forme
buffered aqueous solution
Poids mol.
antigen ~35 kDa
Espèces réactives
human
Technique(s)
immunohistochemistry: 1:250 using human cerebral cortex sections
western blot: 1:250-1:500 using PC-12 cell lysate
Numéro d'accès UniProt
Conditions d'expédition
dry ice
Température de stockage
−20°C
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... EIF2S1(1965)
Description générale
The eukaryotic translation initiation factor 2 subunit α (EIF2A) gene is mapped to human chromosome 14q23.3. The eukaryotic translation initiation factor 2 (eIF2), a multimeric protein is composed of three subunits termed α, β, and γ.
Spécificité
Anti-eIF2α (also known as eIF-2A) recognizes human eIF2α.
Application
Anti-eIF2α antibody produced in rabbit has been used in western blotting.
Anti-elF2α antibody is also suitable for immunohistochemistry at a working dilution of 1:250 using human cerebral cortex sections.
Anti-elF2α antibody is also suitable for immunohistochemistry at a working dilution of 1:250 using human cerebral cortex sections.
Anti-eIF2α antibody produced in rabbit is suitable for immunoblotting at a working dilution of 1:250-1:500 using PC-12 cell lysate. It was used as a primary antibody to detect eIF2α in a study.
Actions biochimiques/physiologiques
Increased eIF2α phosphorylation has been observed in Alzheimer′s disease. Viral infections are also known to induce eIF2α phosphorylation.
The main role of eIF2 complex is to transfer Met-tRNAi in the presence of GTP to the 40S ribosomal subunit during the initiation of translation. Regulation of the initiation of translation at the level of eIF2 involves the phosphorylation of eIF2α at a conserved Ser51 residue. This leads to inhibition of eIF2β activity, thus reducing guanine nucleotide exchange and consequently, the translation rate. Protein synthesis inhibition at the level of eIF2α phosphorylation is linked to a variety of cell stress signaling pathways via specific kinases. Phosphorylation of eIF2α reduces the rate of translation and gives the cell time to correct the stress damage, and selectively enhance the translation of genes important for stress remedy.
Forme physique
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Stockage et stabilité
For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Réf. du produit
Description
Tarif
Produit(s) apparenté(s)
Code de la classe de stockage
10 - Combustible liquids
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
R C Wek et al.
Biochemical Society transactions, 34(Pt 1), 7-11 (2005-10-26)
In response to environmental stresses, a family of protein kinases phosphorylate eIF2 (eukaryotic initiation factor 2) to alleviate cellular injury or alternatively induce apoptosis. Phosphorylation of eIF2 reduces global translation, allowing cells to conserve resources and to initiate a reconfiguration
R C Wek
Trends in biochemical sciences, 19(11), 491-496 (1994-11-01)
Phosphorylation of eukaryotic initiation factor-2 (eIF-2) is an important mechanism regulating general translation initiation. Two mammalian eIF-2 kinases, the double-stranded-RNA-dependent kinase (PKR) and heme-regulated inhibitor kinase (HRI), have been characterized by sequencing, revealing shared sequence and structural features distinct from
Christopher G Proud
Seminars in cell & developmental biology, 16(1), 3-12 (2005-01-22)
Eukaryotic initiation factor eIF2 and its 'exchange factor' eIF2B play a key role in the regulation of protein synthesis in eukaryotes from yeast to mammals. Phosphorylation of eIF2 inhibits eIF2B and thus translation initiation. Four eIF2 kinases are now known
Rita Spilka et al.
Cancer letters, 340(1), 9-21 (2013-07-09)
Eukaryotic gene expression is a complicated process primarily regulated at the levels of gene transcription and mRNA translation. The latter involves four main steps: initiation, elongation, termination and recycling. Translation regulation is primarily achieved during initiation which is orchestrated by
Anton A Komar et al.
International journal of molecular sciences, 21(6) (2020-03-21)
Initiation of protein synthesis in eukaryotes is a complex process requiring more than 12 different initiation factors, comprising over 30 polypeptide chains. The functions of many of these factors have been established in great detail; however, the precise role of
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