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D8156

Sigma-Aldrich

Monoclonal Anti-Digoxin antibody produced in mouse

clone DI-22, ascites fluid

Synonyme(s) :

Monoclonal Anti-Digoxin

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.43

Source biologique

mouse

Niveau de qualité

200

Conjugué

unconjugated

Forme d'anticorps

ascites fluid

Type de produit anticorps

primary antibodies

Clone

DI-22, monoclonal

Contient

15 mM sodium azide

Technique(s)

dot blot: suitable
flow cytometry: suitable
indirect ELISA: 1:10,000 using digoxin-BSA
indirect ELISA: 1:2,500 using digoxigenin-transferrin

Isotype

IgG1

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

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Catégories apparentées

Description générale

Monoclonal Anti-Digoxin (mouse IgG1 isotype) is derived from the hybridoma DI-22 produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with digoxin- keyhole limpet hemocyanin (KLH). Digoxin is obatained from the leaves of the Digitalis lanata, a foxglove plant. The digoxin molecule is made up of a sugar and a cardenolide. It has a molecular weight is 780.95 Da. It is a odorless white crystal, which is soluble in alcohol and freely soluble in pyridine. It is expressed in the duodenum, kidneys, liver and the blood-brain barrier.

Spécificité

Antibody is specific for digoxin and shows a high affinity for digoxigenin.
Monoclonal Anti-Digoxin antibody is specific for digoxin and digoxin-labeled compounds, and shows strong cross-reactivity with digoxigenin.

Immunogène

Digoxin-KLH.

Application

Monoclonal Anti-Digoxin antibody produced in mouse has been used in:
  • enzyme-linked immunosorbent assay (ELISA)
  • dot blot
  • flow cytometry
  • fluorescence in situ hybridization (FISH)
  • DNA hybridization
  • in-situ hybridization (ISH)
  • immunodetection of the probe and chromosome X

Actions biochimiques/physiologiques

Digoxin is a phytoestrogen that binds estrogen receptors and produces inotropic effects in cardiac muscle. Studies show that digoxin decreases the risk of breast and uterus cancers that are estrogen sensitive.
Digoxin is a substrate for P-glycoprotein (P-gp), a membrane efflux transporter. This antibody may be used to detect digoxin-labeled compounds such as oligonucleotides, antibodies or peptides.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

nwg

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Hesed M Padilla-Nash et al.
Nature protocols, 1(6), 3129-3142 (2007-04-05)
Classical banding methods provide basic information about the identities and structures of chromosomes on the basis of their unique banding patterns. Spectral karyotyping (SKY), and the related multiplex fluorescence in situ hybridization (M-FISH), are chromosome-specific multicolor FISH techniques that augment
GSTT1-dependent induction of centromere-negative and-positive micronuclei by l, 2: 3, 4-diepoxybutane in cultured human lymphocytes
Vlachodimitropoulos D, et al.
Mutagenesis, 12(5), 397-403 (1997)
Waqar Ahmed et al.
Scientific reports, 8(1), 15438-15438 (2018-10-20)
Epstein-Barr virus-encoded RNAs (EBER1 and EBER2) are two highly abundant, non-protein coding RNAs consistently expressed in all EBV infected cells, but their function remains poorly understood. Conventional in situ hybridization studies have indicated that these RNAs are present exclusively in
Ying-Liang Duan et al.
Journal of virology, 88(7), 3861-3873 (2014-01-24)
After infection, human cytomegalovirus (HCMV) persists for life. Primary infections and reactivation of latent virus can both result in congenital infection, a leading cause of central nervous system birth defects. We previously reported long-term HCMV infection in the T98G glioblastoma
Digoxin
Small Animal Clinical Pharmacology, 805-806 (2009)
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