D5444

Anti-DCP1A (C-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

• Synonyme(s) : Anti-DCP1 Decapping enzyme 1, homolog A, Anti-SMAD4IP1, Anti-SMIF, Anti-Smad 4-interacting transcription factor, Anti-Smad 4-interacting transcriptional co-activator
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Conjugué: unconjugated
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Forme: buffered aqueous solution
• Poids mol.: antigen ~70 kDa
• Espèces réactives: mouse, human, rat (predicted)
• Concentration: ~1.0 mg/mL
• Technique(s): immunoprecipitation (IP): 5-10 μg using cell lystes of HEK-293T; indirect immunofluorescence: 2-5 μg/mL using paraformaldehyde-fixed NIH-3T3 cells over-expressing human DCP1A or using paraformaldehyde-fixed HEPG2 cells; western blot: 1-2 μg/mL using cell lysates of HEK-293T
• Numéro d'accès UniProt: Q9NPI6
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... DCP1A(55802) ; mouse ... Dcp1a(75901) ; rat ... Dcp1a(361109)

Description

Description générale

Dcp1 colocalizes with Dcp2 in distinct cytoplasmic foci along with other proteins involved in the 5′ to 3′ mRNA decay. These foci are termed PB (processing bodies) or DCP-bodies. Anti-DCP1A (C-terminal) is produced in rabbit using as immunogen a synthetic peptide corresponding to a sequence at C-terminal of human DCP1A conjugated to KLH. Two distinct genes of human DCP1 were identified, DCP1A and DCP1B, which share ~70% homology in their N-terminal and ~30% homology in their full length.

Application

Anti-DCP1A antibody produced in rabbit is suitable for immunoprecipitation at a working concentration of 5-10 μg using cell lystes of HEK-293T, indirect immunofluorescence at 2-5 μg/mL using paraformaldehyde-fixed NIH-3T3 cells over-expressing human DCP1A or using paraformaldehyde-fixed HEPG2 cells and western blot analysis at 1-2 μg/mL working concentration using cell lysates of HEK-293T. It was used as a primary antibody at a working dilution of 1:200 in the immunofluorescence experiment of HeLa cells treated with 5-fluorouracil to study the assembly of stress granules based on RNA incorporation.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence-cell culture cells (1 paper)

Actions biochimiques/physiologiques

Dcp1 cleaves the m7G mRNA cap in the 5′ to 3′ mRNA decay pathway, in association with Dcp2 and Hedls complex. Decapping is a critical and highly regulated step in the turnover of mRNA which involves decapping enzymes that hydrolyze the cap structure at the 5′ mRNA.

Forme physique

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)