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D2191

Sigma-Aldrich

Monoclonal Anti-DC-SIGN1 antibody produced in mouse

clone 120507, purified immunoglobulin, lyophilized powder

Synonyme(s) :

Anti-CD209, Anti-Dendritic Cell-Specific ICAM-3 Grabbing Non-Integrin

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About This Item

Numéro MDL:
MFCD03701938
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

mouse

Niveau de qualité

200

Conjugué

unconjugated

Forme d'anticorps

purified immunoglobulin

Type de produit anticorps

primary antibodies

Clone

120507, monoclonal

Forme

lyophilized powder

Espèces réactives

human

Technique(s)

flow cytometry: 2.5 μg using 106 cells
immunocytochemistry: 8-25 μg/mL
immunohistochemistry: 8-25 μg/mL using fixed cells and tissue sections
western blot: 1 μg/mL

Isotype

IgG2b

Numéro d'accès UniProt

Q9NNX6

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... CD209(30835)

Description générale

DC-SIGN is a type II protein C-type lectin that binds mannose-rich molecules such as ICAM-3 and ICAM-2 in a calcium dependent manner. DC-SIGN, a 44 kDa molecule, is identical to the HIV-1 binding protein.

Spécificité

Recognizes human DC-SIGN on transfected cells and on monocyte derived dentritic cells. The antibody does not react with either parental mouse cells or irrelevant transfectants, such as human DC-SIGN2.

Immunogène

purified from NIH/3T3 cells transfected with recombinant human DC-SIGN.

Application

Monoclonal Anti-DC-SIGN1 antibody produced in mouse is suitable for flow cytometry, immunocytochemistry at a working concentration of 8-25 μg/mL, immunohistochemistry at 8-25 μg/mL using fixed cells and tissue sections and western blotting at 1 μg/mL.

Actions biochimiques/physiologiques

DC-SIGN1 is involved in molecular interactions between dendritic cells and resting T-cells. DC-SIGN interacts with viruses such as X4, R5, X4R5 HIV-1 strains, HIV-2, and SIV. It captures HIV-1 through its interaction with the gp120 envelope glycoprotein and promotes efficient infection of CD4+ T cells that co-express chemokine receptors.

Forme physique

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline with 5% trehalose.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

S Pöhlmann et al.
Journal of virology, 75(10), 4664-4672 (2001-04-20)
Dendritic cells (DCs) efficiently bind and transmit human immunodeficiency virus (HIV) to cocultured T cells and so may play an important role in HIV transmission. DC-SIGN, a novel C-type lectin that is expressed in DCs, has recently been shown to
T B Geijtenbeek et al.
Cell, 100(5), 575-585 (2000-03-18)
Contact between dendritic cells (DC) and resting T cells is essential to initiate a primary immune response. Here, we demonstrate that ICAM-3 expressed by resting T cells is important in this first contact with DC. We discovered that instead of
T B Geijtenbeek et al.
Cell, 100(5), 587-597 (2000-03-18)
Dendritic cells (DC) capture microorganisms that enter peripheral mucosal tissues and then migrate to secondary lymphoid organs, where they present these in antigenic form to resting T cells and thus initiate adaptive immune responses. Here, we describe the properties of
T B Geijtenbeek et al.
Nature immunology, 1(4), 353-357 (2001-03-23)
Dendritic cells (DCs) are recruited from blood into tissues to patrol for foreign antigens. After antigen uptake and processing, DCs migrate to the secondary lymphoid organs to initiate immune responses. We now show that DC-SIGN, a DC-specific C-type lectin, supports
B M Curtis et al.
Proceedings of the National Academy of Sciences of the United States of America, 89(17), 8356-8360 (1992-09-01)
The binding of the human immunodeficiency virus (HIV) envelope glycoprotein gp120 to the cell surface receptor CD4 has been considered a primary determinant of viral tropism. A number of cell types, however, can be infected by the virus, or bind
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