NCI-H69/CPR

96042328, human lung, Not specified

• Code UNSPSC : 41106514

Propriétés

• Nom du produit: NCI-H69/CPR, 96042328
• Source biologique: human lung
• Mode de croissance: Aggregates in suspension
• Caryotype: Not specified
• Morphologie: Not specified
• Produits: Not specified
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): cancer
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Human small cell lung cancer, drug-resistant

Description de la lignée cellulaire

NCI-H69/CPR is a drug resistant subline of NCI-H69 (Sigma Catalogue number. 91091802). Drug resistance was developed by addition of a stepwise increase in cisplatin to the growth medium of the parental line. The cell line exhibits a 5-fold resistance to cisplatin and is cross resistant to melphalan. A significant change in cellular glutathione content or sensitivity to cadmium chloride (as indicator of metallothionein content) was not detected, but changes in glutathione-S-transferase activity were seen. Cisplatin accumulation was unchanged compared with the parent line. It is recommended to culture the cells without drugs after resuscitation until the first passage.

Application

Drug-resistance studies, small cell lung cancer

Milieu de culture

RPMI 1640 + 2mM Glutamine + 0.4μg/ml cisplatin + 10% Foetal Bovine Serum (FBS).

Procédure de repiquage

Maintain cultures between 3-9 x 100,000 cells/ml; 5% CO₂; 37ºC; resuscitate in drug-free medium until first passage.

Autres remarques

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