SR-4987

94102704, mouse bone, Fibroblast-like

• Code UNSPSC : 41106514

Propriétés

• product name: SR-4987, 94102704
• Source biologique: mouse bone
• Mode de croissance: Adherent
• Caryotype: Not specified
• Morphologie: Fibroblast-like
• Produits: Macrophage colony stimulating factor (M-MCF)
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Mouse bone marrow stromal cells

Description de la lignée cellulaire

A bone marrow-derived stromal cell line established by A Pessina in 1987 from a normal female 5 month old mouse. A bone marrow culture was treated with the supernatant from Y1 cells which actively produce viral C-particles (MuLV). This fibroblast-like cell line produces macrophage-colony stimulating factor (M-MCF) and exhibits a high sensitivity to fibroblast growth factor (FGF) in clonogenic assays. The cells produce sarcomas in syngenic mice and have a reported doubling time of 14.7 hours.

Milieu de culture

McCoy′s 5a + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:4 to 1:8 i.e. seeding at 1-2x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Autres remarques

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