DBTRG.05MG

93061119, human brain, Glial

• Code UNSPSC : 41106514

Propriétés

• product name: DBTRG.05MG, 93061119
• Source biologique: human brain
• Mode de croissance: Adherent
• Caryotype: 2n = 87-91, hypotetraploid
• Morphologie: Glial
• Produits: Not specified
• Récepteurs: Epidermal growth factor (EGF) R
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): cancer
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Human glioblastoma

Description de la lignée cellulaire

Derived from an adult female with glioblastoma multiforme treated with local brain irradiation and multi drug chemotherapy. By flow cytometry these cells were almost 100% positive for MHC Class I.

Application

Tumourigenicity studies

Profil d'ADN

STR-PCR Data: Amelogenin: X
CSF1PO: 10,11
D13S317: 9
D16S539: 12
D5S818: 12,13
D7S820: 11
THO1: 8
TPOX: 8
vWA: 15,16

Milieu de culture

RPMI 1640 + 2mM Glutamine + 1% HT + 1mM Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS).

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Autres remarques

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