C0111
93012123, from human blood
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About This Item
Code UNSPSC :
41106514
Produits recommandés
Source biologique
human blood
Numéro de panel HRC
2
Ethnicité
UK Caucasian
Sexe
female
Mode de croissance
Suspension
Caryotype
Not Specified
Morphologie
Lymphoblastoid
Technique(s)
cell culture | mammalian: suitable
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Origine de la lignée cellulaire
UK Caucasian Female
Description de la lignée cellulaire
A B-lymphoblastoid cell line derived from a randomly selected Caucasian blood donor whose parents and grandparents were born in the UK or Ireland. The cell line was generated by Epstein-Barr Virus (EBV) transformation of peripheral blood lymphocytes.
This cell line is part of the European Collection of Cell Cultures (ECACC) Human Random Control (HRC) collection. The HRC collection consists of more than 700 B-lymphoblastoid cell lines derived from randomly selected Caucasian blood donors whose parents and grandparents were born in the UK or Ireland. The HRC collection can be used as a renewable control population for genetic research.
This cell line is part of the European Collection of Cell Cultures (ECACC) Human Random Control (HRC) collection. The HRC collection consists of more than 700 B-lymphoblastoid cell lines derived from randomly selected Caucasian blood donors whose parents and grandparents were born in the UK or Ireland. The HRC collection can be used as a renewable control population for genetic research.
Application
As part of the Human Random Control collection this cell line can be used as a renewable source of genomic DNA reference material for a wide range of genetic research applications.
Milieu de culture
RPMI 1640 (R0883) + 2 mM L-Glutamine (G7513) + 20% FBS / FCS (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.
Procédure de repiquage
Maintain cultures between 3 x 105 - 2 x 106 cells/ml; 5% CO2, 37 °C.
Autres remarques
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