FRTL-5

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 91030711, rat thyroid, Not specified

• Code UNSPSC : 41106514

Propriétés

• Source biologique: rat thyroid
• Conditionnement: tube of 5 μg 91030711-DNA-5UG; pkg of vial of cells 91030711-1VL
• Mode de croissance: Adherent aggregates
• Caryotype: Not specified
• Morphologie: Not specified
• Produits: Thyroglobulin
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Rat thyroid

Description de la lignée cellulaire

This thyroid cell line has been established from normal thyroid glands from 5 to 6-week-old Fischer rats. FRTL-5 is a clonal line of the continuously cultured FRTL cells derived by successive clonal isolation. The cells are characterised by maintaining highly differentiated thyroid features such as secretion of thyroglobulin and concentration of iodide. FRTL-5 cells have been used as assays measuring the stimulatory activity of human autoimmune immunoglobulins from patients with Graves disease. Tend to grow in three-dimensional structures, monolayer not formed.

Application

Study of thyroid cells, hormone dependency and secretion.

Milieu de culture

Coon′s modified Ham′s F12 medium (or Kaign′s modified Ham′s F12 + 45mg/L ascorbic acid + 18mg/L Inositol) + 2mM Glutamine + 10μg/ml Insulin + 10nM Hydrocortisone + 5μg/ml Transferrin + 10ng/ml gly-his-lys acetate + 10ng/ml somatostatin + 5% Foetal Bovine Serum (FBS) + 10mU/ml TSH. As an alternative to Coon′s modified Ham′s F12, it is possible to use Kaign′s modified Ham′s F12 supplemented with 45mg/L Ascorbic acid and 18mg/L Inositol.

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Autres remarques

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