G-402

90112715

• Code UNSPSC : 41106514

Propriétés

• Source biologique: human kidney
• Mode de croissance: Adherent
• Caryotype: Modal no. 47
• Morphologie: Epithelial-like
• Produits: Not specified
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): cancer
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Human Caucasian renal leiomyoblastoma

Description de la lignée cellulaire

Derived from a tumour of a 9 month old female Caucasian. Highly transformed and grows in soft agar.

Application

Transformation and tumourigenicity studies

Profil d'ADN

STR-PCR Data: Amelogenin: X
CSF1PO: 10,13
D13S317: 9,12
D16S539: 11,13
D5S818: 11,12
D7S820: 9,10
THO1: 7,9
TPOX: 10
vWA: 13,17

Milieu de culture

McCoy′s 5a + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-5x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Change medium every 4 days.

Autres remarques

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