LL47 (MaDo)

90102538, human lung, Fibroblast-like

• Code UNSPSC : 41106514

Propriétés

• product name: LL47 (MaDo), 90102538
• Source biologique: human lung
• Mode de croissance: Adherent
• Caryotype: 2n = 46; diploid
• Morphologie: Fibroblast-like
• Produits: Not specified
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Human diploid lung

Description de la lignée cellulaire

Derived from normal lung tissue obtained from a 16 year old male with osteogenic sarcoma. Ethnicity: Black.

Application

Tumourigenicity studies

Profil d'ADN

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 7,12
D13S317: 9,11
D16S539: 11
D5S818: 12,13
D7S820: 8,10
THO1: 7,9.3
TPOX: 9,10
vWA: 14,16

Milieu de culture

Coon′s modified Ham′s F12 + Kaign′s modified Ham′s F12 + 45mg/L ascorbic acid + 18mg/L Inositol + 2mM Glutamine + 15% Foetal Bovine Serum (FBS).

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 3x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C. A further 15 population doublings expected.

Autres remarques

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