RR1022

90102526, rat unknown/unspecified, Epithelial

• Code UNSPSC : 41106514

Propriétés

• Nom du produit: RR1022, 90102526
• Source biologique: rat unknown/unspecified
• Conditionnement: tube of 5 μg 90102526-DNA-5UG; pkg of vial of cells 90102526-1VL
• Mode de croissance: Adherent
• Caryotype: 2n = 42
• Morphologie: Epithelial
• Produits: Not specified
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): cancer
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Rat Amsterdam Schmidt-Ruppin sarcoma

Description de la lignée cellulaire

Derived from a Rous sarcoma virus (Schmidt-Ruppin strain) induced tumour in an inbred Amsterdam rat. The cells are highly tumorigenic in new-born rats and also chickens. There is some evidence that the cell line is latently infected with the virus.

Application

Tumourigenicity and virus studies: Rous sarcoma virus

Milieu de culture

Medium 199 (HBSS) + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C.

Autres remarques

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