NB4 1A3

89121405, mouse nerve, Neuroblast

• Code UNSPSC : 41106514

Propriétés

• product name: NB4 1A3, 89121405
• Source biologique: mouse nerve
• Mode de croissance: Adherent
• Caryotype: Unstable
• Morphologie: Neuroblast
• Produits: Acetylcholinesterase, choline acetylase, tyrosine hydroxylase
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): cancer
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Mouse C-1300 Neuroblastoma

Description de la lignée cellulaire

Cloned from the C1300 mouse neuroblastoma line. Adapted to culture by alternate in vivo and in vitro passage. Cells have characteristics of mature neurons and secrete the enzymes for the synthesis of the neurotransmitters, choline acetylase and tyrosine hydroxylase. Cells are susceptible to VSV, HSV and Vaccinia viruses.

Application

Tumourigenicity, enzymatic and virus studies: VSV, HSV, vaccinia virus

Milieu de culture

Ham′s F10 + 2mM Glutamine + 15% Horse Serum (HS) + 2.5% Foetal Bovine Serum (FBS).

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 3-5x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C.

Autres remarques

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