Clone C6/36

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 89051705, insect larvae, Not specified

• Code UNSPSC : 41106514

Propriétés

• Source biologique: insect larvae
• Conditionnement: tube of 5 μg 89051705-DNA-5UG; pkg of vial of cells 89051705-1VL
• Mode de croissance: Adherent
• Caryotype: Diploid
• Morphologie: Not specified
• Produits: Not specified
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Mosquito larvae, Aedes albopictus

Description de la lignée cellulaire

Derived from Aedes albopictus cells. Useful for propagation of Dengue virus and flaviviruses. Cells are non-anchorage dependant, not tumourigenic and maintain a diploid chromosome number.

Application

Clone C6/36 has been used to screen anti-Wolbachia compounds. It has also been used to study the effects of tick-borne encephalitis virus (TBEV) vaccine.

Milieu de culture

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS) (Sodium Bicarbonate (NaHCO₃) level of 1.1g/l is recommended).

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 5000 - 30000 cells/cm² . Cells are dislodged by mechanical agitation; 5% CO₂; 28°C. Freeze in 10% glycerol.

Autres remarques

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