H4II

89042702

• Code UNSPSC : 41106514

Propriétés

• Source biologique: rat skin
• Mode de croissance: Adherent
• Caryotype: 52 chromosomes
• Morphologie: Epithelial
• Produits: Albumin, transferrin, prothrombin
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): cancer
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Rat skin Hepatocellular carcinoma

Description de la lignée cellulaire

From H-35 Reuber hepatoma induced by chemical carcinogenesis in an AxC rat . Tyrosine aminotransferase positive. Synthesises several serum proteins including albumin, transferrin and prothrombin. (Same as H4-11-E-C3 (Sigma Catalogue number. 85061112)).

Application

Gene expression analysis.

Milieu de culture

Coon′s modified Ham′s F12 (or Kaign′s modified Ham′s F12 + 45mg/L ascorbic acid + 18mg/L Inositol) + 2mM Glutamine + 5% Foetal Bovine Serum (FBS).

Procédure de repiquage

Split semi-confluent [60%-70%] cultures 1:100 i.e. seeding at 2x100 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Saturation density is reached at 15x1000,000 cells/10cm² dish.

Autres remarques

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