HL60 Ast.3

88120801, human blood, Lymphoblastoid

• Code UNSPSC : 41106514

Propriétés

• product name: HL60 Ast.3, 88120801
• Source biologique: human blood
• Mode de croissance: Suspension
• Caryotype: Modal no. = 93, Hypertetraploid
• Morphologie: Lymphoblastoid
• Produits: Not specified
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): cancer
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Human Caucasian promyelocytic leukaemia

Description de la lignée cellulaire

Variant of the promyelocytic cell line HL60 isolated after treating cells with α-particle radiation and then cloned in medium containing 1.25% DMSO. Cells are capable of differentiating into neutrophils by induction with 1.75% DMSO.

Application

Differentiation studies

Profil d'ADN

STR-PCR Data: Amelogenin: X
CSF1PO: 14
D13S317: 8,11
D16S539: 11
D5S818: 12
D7S820: 11,12
THO1: 7
TPOX: 8,11
vWA: 16

Milieu de culture

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + 1.25% Dimethyl Sulphoxide (DMSO).

Procédure de repiquage

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C. Do not allow the culture medium to become acidic or the cell line will differentiate.

Autres remarques

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