RFL-6

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 86072501, rat lung, Fibroblast

• Code UNSPSC : 41106514

Propriétés

• Source biologique: rat lung
• Conditionnement: tube of 5 μg 86072501-DNA-5UG; pkg of vial of cells 86072501-1VL
• Mode de croissance: Adherent
• Caryotype: 2n = 42
• Morphologie: Fibroblast
• Produits: Not specified
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Rat Sprague-Dawley lung

Description de la lignée cellulaire

Derived from the lung tissue of a normal germ-free, 18 day gestation, Sprague-Dawley rat foetus. At least 45 population doublings expected.

Milieu de culture

Ham′s F12 + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 20% Foetal Bovine Serum (FBS).

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-5x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Autres remarques

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