SC-1

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 86060301, mouse embryo, Fibroblast

• Code UNSPSC : 41106514

Propriétés

• Source biologique: mouse embryo
• Conditionnement: tube of 5 μg 86060301-DNA-5UG; pkg of vial of cells 86060301-1VL
• Mode de croissance: Adherent
• Caryotype: Not specified
• Morphologie: Fibroblast
• Produits: Not specified
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Mouse feral embryo

Description de la lignée cellulaire

Sensitive to both N- and B- trophic viruses. Cells exhibit a greater sensitivity than other mouse lines to MuLV strains and to a limited extent support the replication of xenotropic MuLV. This cell line has been mycoplasma eradicated at ECACC.

Application

Virus studies: N- and B- trophic viruses, MuLV

Milieu de culture

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-5x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Autres remarques

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