OACM5.1 C cell line human

11012006, human esophagus (lung metastasis). Floating spheroids, some fibroblastoid morphology

• Synonyme(s) : Cell culture
• Code UNSPSC : 41106514

Propriétés

• product name: OACM5.1 C cell line human, 11012006
• Source biologique: human esophagus (lung metastasis)
• Mode de croissance: Adherent
• Caryotype: Hypodiploid 39-41 chromosomes
• Morphologie: Multicellular floating spheroids some with fibroblastoid morphology
• Produits: P53 mutation c.164delC c.743G>A
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): metastasis
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Barretts adenocarcinoma, adenocarcinoma of distal oesophagus

Application

Recommended culture medium: RPMI 1640 + 2mM L-Glutamine + 10% FBS

Profil d'ADN

STR-PCR Data: Amelogenin: X
CSF1PO: 10,13
D13S317: 11,12
D16S539: 10,11
D5S818: 11,12
D7S820: 8.2,8.2
THO1: 6,9.3
TPOX: 8
vWA: 19,20

Milieu de culture

RPMI-1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 3 x 10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Autres remarques

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