BRIN-BG7

10020902, rat pancreas (islets), Epitheloid

• Synonyme(s) : BRIN
• Code UNSPSC : 41106514

Propriétés

• product name: BRIN-BG7, from rat pancreas(islets), 10020902
• Source biologique: rat pancreas (islets)
• Mode de croissance: Adherent
• Caryotype: Modal chromosome number 66 up to passage 50
• Morphologie: Epitheloid
• Produits: BRIN-BG7 is a stable, glucose-responsive insulin secreting beta cell line expressing insulin, glucokinase and IAPP as detected by immunocytochemistry. BRIN-BG7 cells express the GLUT2 glucose transporter.
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Conditions d'expédition: dry ice

Description

Origine de la lignée cellulaire

A rat pancreatic Beta cell: RINm5F hybrid cell line. Insulin-secreting cell line

Profil d'ADN

Not specified

Milieu de culture

RPMI-1640 + 2 mM Glutamine + 10% FCS / FBS

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:8 to 1:10 , i.e., seeding at 2-4 x 10⁴ cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37 °C. Population doubling approx 20hrs. At confluence 10⁵ cells/cm² can be expected.

Autres remarques

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Clause de non-responsabilité

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.