GPNT

9061601, rat brain, Fusiform

• Code UNSPSC : 41106514

Propriétés

• product name: GPNT, from rat brain, 09061601
• Source biologique: rat brain
• Mode de croissance: Adherent
• Caryotype: Not specified
• Morphologie: Fusiform
• Produits: GPNT cells express tight junction proteins
• Récepteurs: P-glycoprotein, PECAM-1, ICAM-1, VCAM-1 Glut-1, transferrin receptor, MHC class I
• Technique(s): cell culture | mammalian: suitable
• Conditions d'expédition: dry ice

Description

Origine de la lignée cellulaire

An immortalised Lewis rat brain vascular endothelial cell line

Description de la lignée cellulaire

GPNT was derived from the immortalised rat brain endothelial cell line GP8 by lipofectin-mediated transfection with a vector containing the puromycin resistance gene. GPNT exhibits a non-transformed phenotype.

Profil d'ADN

Not specified

Milieu de culture

Hams F10 + 10% Heat-inactivated FCS + 2 mM Glutamine + 2 ng/ml basic fibroblast growth factor + 80 μg/ml heparin. All plasticware is pre-coated for several hours with collagen (Type I from calf skin) at 10μg/cm2 from a 0.01% solution in acetic acid. After removal the coated plasticware is exposed to a mMonia vapour for 5 min. Plasticware must be rinsed thoroughly with PBS prior to use.

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:4 to 1:10 , i.e., seeding at 3 x 10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37 °C. Population doubling approx 10hrs.

Autres remarques

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Clause de non-responsabilité

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.