N1E-115-1

8062511, mouse nerve, neuronal

• Code UNSPSC : 41106514

Propriétés

• product name: N1E-115-1, 08062511
• Source biologique: mouse nerve
• Mode de croissance: Adherent
• Caryotype: modal no. of chromosomes is 192 see reference
• Morphologie: neuronal
• Produits: Not specified
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): cancer
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Mouse neuroblastoma

Description de la lignée cellulaire

A subclone of the cell line N1E-115 established by Amano et al. The cell line N1E-115 is an adrenergic cell line derived from mouse neuroblastoma C1300. (Mouse strain A/Jax). N1E-115 cells are reported to be slightly contaminated with with a cell type of the morphology of C6 glioma cells. It is recommended to use N1E-115-1, a subclone of N1E-115, that is devoid of the contamination.

Application

can be used as a neuronal model

Milieu de culture

DMEM + 2 mM Glutamine + 10% Foetal Bovine Serum (FBS)

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.05% trypsin/EDTA; 5% CO₂; 37°C. Population doubling approximately 20 hours.

Autres remarques

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