C8511

Cathepsin C from bovine spleen

Type X, lyophilized powder, ≥5 units/mg protein

• Synonyme(s) : Dipeptidyl aminopeptidase, Dipeptidyl peptidase I
• Numéro CAS: 9032-68-2
• Numéro de classification (Commission des enzymes): 3.4.14.1 (BRENDA, IUBMB)
• Numéro CE : 232-881-4
• Numéro MDL: MFCD00081490
• Code UNSPSC : 12352204
• Nomenclature NACRES : NA.54

Propriétés

• Type: Type X
• Niveau de qualité: 200
• Forme: lyophilized powder
• Activité spécifique: ≥5 units/mg protein
• Composition: Protein, ≥25% biuret
• Conditions d'expédition: dry ice
• Température de stockage: −20°C

Description

Application

Cathepsin C has been used in a study that demonstrated the potential of a proteomics approach to identify novel proteins expressed by extravillous trophoblast and to uncover the mechanisms leading to disease states in pregnancy. Cathepsin C has also been used in a study to evaluate biodegradable thermogels.

The enzyme from Sigma has been used in the activation of granzyme k (Gzmk) precursor from E. coli. Granzymes are granule-stored lymphocyte serine proteases that are implicated in T- and natural killer cell-mediated cytotoxic defense reactions.

Actions biochimiques/physiologiques

Cathespin C is a dipeptidyl aminopeptidase that can sequentially remove dipeptides from a peptide chain with an unsubstituted N-terminus. The enzyme exhibits a preference for glycine and proline as N-terminal aminoacids. Substrates that have an N-terminal lysyl or arginyl residue, or a penultimate proryl residue are not targeted by this enzyme. The endopeptidase activity requires the presence of halide ions and sulfydryl activators.

Attention

Unstable. Keep frozen.

Définition de l'unité

One unit will produce 1 μmole of Gly-Phe-NHOH from Gly-Phe-NH₂ and hydroxylamine per min at pH 6.8 at 37 °C using DL-phenylalanine hydroxamic acid as the standard. In addition to its hydrolytic properties, cathepsin C catalyzes the polymerization of dipeptide amides.

Forme physique

Lyophilized from a 1 M sodium chloride solution.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)