C5301
Monoclonal Anti-Cytokeratin Peptide 8 antibody produced in mouse
clone M20, ascites fluid
Synonyme(s) :
Anti-CARD2, Anti-CK-8, Anti-CK8, Anti-CYK8, Anti-K2C8, Anti-K8, Anti-KO
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About This Item
Produits recommandés
Source biologique
mouse
Niveau de qualité
Conjugué
unconjugated
Forme d'anticorps
ascites fluid
Type de produit anticorps
primary antibodies
Clone
M20, monoclonal
Poids mol.
antigen 52 kDa
Contient
15 mM sodium azide
Espèces réactives
feline, human, rabbit, canine, bovine
Technique(s)
immunohistochemistry (frozen sections): suitable
indirect immunofluorescence: 1:200 using frozen sections of human or animal tissue
western blot: suitable
Isotype
IgG1
Numéro d'accès UniProt
Conditions d'expédition
dry ice
Température de stockage
−20°C
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... KRT8(3856)
Description générale
Cytokeratin is a 52.5kD cytoplasmic structural protein that belongs to type II, neutral-to-basic subfamily. It consists of 29 different proteins which is the characteristic of epithelial and trichocytic cells. Monoclonal anti-cytokeratin peptide 8 antibody can be used as a marker for epithelial cell differentiation as well as a tool for tumor identification and classification. It can also be used in western blotting. Mouse anti-cytokeratin peptide 8 antibody reacts specifically with various human simple and complex epithelia like- liver, intestine, pancreas, urinary bladder, salivary gland, thyroid, prostate, mesothel, and placenta but not with stratified squamous epithelia. The product also does not react with non-epithelial tissues (exceptions-certain smooth muscle cells). This product has also shown cross reactivity for cytokeratin of rabbit, cow, dog, and cat.Monoclonal Anti-Cytokeratin 8 (mouse IgG1 isotype) is derived from the M20 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a cytokeratin preparation purified from the human breast carcinoma cell line MCF7.
Spécificité
Mouse anti-cytokeratin peptide 8 antibody reacts specifically with various human simple and complex epithelia like- liver, intestine, pancreas, urinary bladder, salivary gland, thyroid, prostate, mesothel, and placenta but not with stratified squamous epithelia. The product also does not react with non-epithelial tissues (exceptions-certain smooth muscle cells). This product has also shown cross reactivity for cytokeratin of rabbit, cow, dog, and cat.
Immunogène
cytokeratin from human breast carcinoma cell line MCF7.
Application
Monoclonal anti-cytokeratin peptide 8 antibody has been used in
- immunofluorescence microscopy
- immunohistochemistry
- dot blotting
- protein preparation from bovine epithelial cells
- immunofluorescence
- western blotting
Actions biochimiques/physiologiques
Monoclonal anti-cytokeratin peptide 8 antibody can be used as a marker for epithelial cell differentiation as well as a tool for tumor identification and classification. It can also be used in western blotting.
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Réf. du produit
Description
Tarif
Code de la classe de stockage
10 - Combustible liquids
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
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Myoglobin: a promising exogenous reference marker using in proteomics analysis
Wang T, et al.
Food Science and Biotechnology, 22, 393-398 (2013)
David Soler et al.
Genes, chromosomes & cancer, 44(4), 339-350 (2005-07-30)
The development of genomic instability is an important step toward generating the multiple genetic changes required for cancer. Telomere dysfunction is one of the factors that contribute to tumorigenesis. Telomeres shorten with each cell division in the absence of telomerase.
G N Van Muijen et al.
Laboratory investigation; a journal of technical methods and pathology, 57(4), 359-369 (1987-10-01)
Tissues from human fetuses (12 to 14 weeks) were studied by using immunohistochemical methods, with special emphasis on coexpression of intermediate filaments. Well-characterized antibodies, monoclonal as well as polyclonal were used. Indirect immunoperoxidase staining disclosed simultaneous expression of cytokeratin and
S Portet et al.
Cytometry, 35(3), 203-213 (1999-03-19)
In the MCF7 human breast cancer cell line, several patterns of cytokeratin networks are observed, depending on the intracellular localization. Our hypothesis is that architectural variations of cytokeratin networks depend on local tensions or forces appearing spontaneously in the cytoplasm.
Mingxi Hua et al.
Journal of cell science, 125(Pt 23), 5800-5810 (2012-09-15)
The correct functioning of hepatocytes requires the establishment and maintenance of hepatocyte polarity. However, the mechanisms regulating the generation of hepatocyte polarity are not completely understood. The differentiation of human fetal hepatic progenitor cells (hFHPCs) into functional hepatocytes provides a
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