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Key Documents

C-29912

Sigma-Aldrich

Freezing Medium Cryo-SFM

Serum-free medium for cryopreservation, 125 ml

Synonyme(s) :

Cryopreservation Medium

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About This Item

Code UNSPSC :
12352207
Nomenclature NACRES :
NA.81

Forme

aqueous solution

Conditionnement

pkg of 125 ml

Technique(s)

cell culture | mammalian: suitable

Conditions d'expédition

wet ice

Température de stockage

2-8°C

Catégories apparentées

Description générale

Cryo-SFM

Application

Cryo-SFM is a protein-free, defined and animal component-free cryopreservation medium. The optimized formulation is based on methylcellulose, DMSO and other cryoprotectants for the cryopreservation of human and animal primary cells. Cryo-SFM provides excellent results with all types of cells including primary human cells, stem cells and cell lines. Cells frozen in Cryo-SFM exhibit superior viability, attachment and subsequent growth performance after thawing. The medium is the first choice for all types of primary cells and cell lines.

Qualité

All lots of PromoCell Cryo-SFM are subjected to comprehensive quality control tests. Each lot is routinely tested for its performance with primary human cells, for the absence of cytotoxicity, and for physical parameters such as osmolality and pH level. Approved in-house lots are used as a reference. In addition, all lots of Cryo-SFM have been tested for the absence of microbial contaminants (fungi, bacteria).

Notes préparatoires

Detach adherent cells using the PromoCell DetachKit (C-41200, C-41210, C-41220). Resuspend the cells in cold or room temperature Cryo-SFM at a concentration of 0.1 to 10 million cells/ml. Transfer the cell suspension into cryo-vials, freeze them gradually (-1°C/minute) to -70°C or lower overnight and subsequently store the vials in liquid nitrogen. Note: Each freeze/thaw cycle leads to a loss in proliferation potential and cell number, particularly when working with fragile primary cells. If freezing the cells, please do so at as low a passage number as possible.

Stockage et stabilité

Store Cryo-SFM at 4 to 8°C in the dark immediately after arrival. If stored properly, the product is stable until the expiry date stated on the label.

Procédure de repiquage

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Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

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