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Key Documents

C-26230

Sigma-Aldrich

Renal Epithelial Cell Growth Medium 2

Basal Medium, 500 ml

Synonyme(s) :

Recommended with C-39606 or C-39605

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About This Item

Code UNSPSC :
12352207
Nomenclature NACRES :
NA.81

Forme

aqueous solution

Conditionnement

pkg of 500 ml

Technique(s)

cell culture | mammalian: suitable

Conditions d'expédition

wet ice

Température de stockage

2-8°C

Description générale

Renal Epithelial Cell Basal Medium 2 (supplements sold separately)

To form a complete medium, it should be combined with the media supplements: C-39606 or C-39605

Application

Renal Epithelial Cell Growth Medium 2 is an optimized low-serum (5% V/V) medium that additionally contains Epinephrine. The combination of low serum and selected growth factors ensures successful growth in vitro making the Renal Epithelial Cell Growth Medium 2 the first choice for PromoCell Renal Epithelial Cells. The Renal Epithelial Cell Growth Medium 2 is optimized for primary human cells, but can also be used for porcine and mouse renal epithelial cells.Recommended for:Human Renal Epithelial Cells (HREpC)Human Renal Cortical Epithelial Cells (HRCEpC)

Composants

Part of a Cell Culture System offered in a variety of formats:Growth Medium (Ready-to-use): Includes Basal Medium and SupplementMixGrowth Medium Kit: Includes Basal Medium and SupplementPackSupplementMix: Contains all media supplements premixed in one vialSupplementPack: Contains all media supplements as individual vials

Qualité

All lots of PromoCell Renal Epithelial Cell Media are subjected to comprehensive quality control tests using primary human renal epithelial cells. Each lot is checked for growth promoting activity, adherence rate, and typical morphology of the tested renal epithelial cells. Approved in-house lots of media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).

Stockage et stabilité

Store the Basal Medium at 4 to 8°C in the dark immediately after arrival. Do not freeze the Basal Medium. If stored properly, the medium is stable until the expiry date stated on the label. After adding the supplements to the Basal Medium, the shelf life of the complete medium is 6 weeks at 4 to 8°C. Do not freeze the complete medium. For use, pre-warm only an aliquot of the complete medium and keep the remaining medium refrigerated at 4 to 8°C

Procédure de repiquage

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Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

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Contenu apparenté

Primary Human Renal Epithelial Cells and Growth Media

Primary human renal epithelial cells and low-serum growth medium from PromoCell are optimized for the in vitro cultivation of epithelial cells from the kidney. Protocols for handling, cell passaging, media, and product use.

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