C-12271

Human Aortic Endothelial Cells (HAoEC)

500,000 cryopreserved cells

• Synonyme(s) : HAoEC cells
• Code UNSPSC : 41106514

Propriétés

• Source biologique: human aorta (thoracic and abdominal)
• Conditionnement: pkg of 500,000 cells
• Morphologie: ( endothelial)
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): hypertension; cardiovascular diseases
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Description générale

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Origine de la lignée cellulaire

Aorta

Application

Primary Human Aortic Endothelial Cells (HAoEC) are isolated from the human ascending (thoracic) and descending (abdominal) aorta and are provided in a cryopreserved format. They are useful for studying vascular diseases such as thrombosis, atherosclerosis, and hypertension as well as for stent-graft compatibility testing.

Qualité

Rigid quality control tests are performed for each lot of Endothelial Cells from large vessels. They are tested for cell morphology, adherence rate, and cell viability. Flow cytometric analyses for cell-type specific markers, e.g. von Willebrand Factor (vWF) and CD31, as well as Dil-Ac-LDL uptake assays are also carried out for each lot. Growth performance is tested through multiple passages up to 15 population doublings (PD) under culture conditions without antibiotics and antimycotics. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).

Avertissement

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.

Procédure de repiquage

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Autres remarques

Recommended Plating Density: 5000 - 10000 cells per cm²Passage After Thawing: P2Tested Markers: von Willebrand Factor (vWF) positive, CD31 positive, Dil-Ac-LDL uptake positiveGuaranteed population doublings: >15

Produits recommandés

Recommended Primary Cell Culture Media:Link

Clause de non-responsabilité

Ce produit, destiné à la recherche scientifique, est soumis à une réglementation spécifique en France, y compris pour les activités d′importation et d′exportation (Article L 1211-1 alinéa 2 du Code de la Santé Publique). L′acheteur (c′est-à-dire l′utilisateur FINAL) est tenu d′obtenir une autorisation d′importation auprès du Ministère français de la Recherche, mentionné à l′article L1245-5-1 II du Code de la Santé Publique. En commandant ce produit, vous confirmez détenir l′autorisation d′importation requise.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable