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Key Documents

C0992

Sigma-Aldrich

Monoclonal Anti-Cy3/Cy5 antibody produced in mouse

clone CY-96, purified from hybridoma cell culture

Synonyme(s) :

Monoclonal Anti-Cy3/Cy5, Cy3 Antibody, Cy3 Antibody - Anti-Cy3/Cy5 antibody, Mouse monoclonal

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

mouse

Conjugué

unconjugated

Forme d'anticorps

purified from hybridoma cell culture

Type de produit anticorps

primary antibodies

Clone

CY-96, monoclonal

Forme

buffered aqueous solution

Concentration

~1.5 mg/mL

Technique(s)

direct ELISA: suitable
dot blot: 1-2 μg/mL using cell protein extracts labeded with Cy3 or Cy5
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
microarray: suitable

Isotype

IgG2a

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

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Description générale

Anti-Cy3/Cy5 antibody, Mouse monoclonal, (mouse IgG2a isotype) is derived from the hybridoma CY-96 produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from Balb/c mice immunized with a mixture of proteins labeled with Cy3 or Cy5.

Spécificité

The antibody recognizes Cy3 and Cy5 conjugated to proteins.

Immunogène

mixture of proteins labeled with Cy3/Cy5.

Application

Anti-Cy3/Cy5 antibody, Mouse monoclonal has been used in:
  • immunofluorescence
  • western blot
  • dot blot
  • enzyme linked immunosorbent assay (ELISA)
  • immunoprecipitation
  • immunocytochemistry
  • protein microarrays
  • In in situ hybridization

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)

Actions biochimiques/physiologiques

Cy 3 and Cy5 are the most popular cyanine dyes, used combined for two color detection. Cy3 dyes are fluorescent orange while Cy5 is fluorescent in the red region. Cyanine belonging to polymethine group. CyDyes are a family of fluorophores that can be used for labeling proteins, peptides, DNA, RNA, and other biomolecules. These dyes are small, pH insensitive, soluble in aqueous solution and are tolerant to DMSO. They are more photostable than fluorescein, have high molar extinction coefficients and favorable quantum yields. Mainly Cy3 and Cy5 are used in many different biological assays such as DNA microarrays, protein microarrays, two-dimensional protein analysis (2D gels), fluorescence resonance energy transfer (FRET), and immunocytochemistry.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Chiaki Nagai-Okatani et al.
International journal of molecular sciences, 20(3) (2019-02-10)
Lectin microarray (LMA) is a highly sensitive technology used to obtain the global glycomic profiles of endogenous glycoproteins in biological samples including formalin-fixed paraffin-embedded tissue sections. Here, we describe an effective method for cell type-selective glycomic profiling of tissue fragments
Pier Giorgio Righetti et al.
European journal of mass spectrometry (Chichester, England), 10(3), 335-348 (2004-06-10)
The present review attempts to cover the vast array of methods which have appeared in the last few years for performing quantitative proteome analysis. These methods are divided into two classes: those applicable to conventional two-dimensional map analysis, coupling orthogonally
Myosin-Va mediates RNA distribution in primary fibroblasts from multiple organs
Salerno V P, et al.
Cell Motility and the Cytoskeleton, 65(5), 422-433 (2008)
Transplantation of ovarian granulosa-like cells derived from human induced pluripotent stem cells for the treatment of murine premature ovarian failure
Liu T. et al.
Molecular Medicine Reports, 13(6), 5053-5058 (2016)
G Enders
Acta neurochirurgica. Supplement, 89, 9-13 (2004-09-01)
Microarray analysis has been emerged as a tool to characterize the overall reaction of cells in culture or tissue to different stimuli e.g. stressful events by analysing bulk RNA present at a particular time point. It has supplemented or even
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