C0794
Chitosanase from Streptomyces sp.
buffered aqueous glycerol solution, ≥15 units/mg protein (E1%)
Synonyme(s) :
Chitosan N-acetylglucosaminohydrolase
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About This Item
Numéro CAS:
Numéro MDL:
Code UNSPSC :
12352204
Nomenclature NACRES :
NA.54
Produits recommandés
Forme
buffered aqueous glycerol solution
Niveau de qualité
Activité spécifique
≥15 units/mg protein (E1%)
Concentration
≥0.5 mg/mL
Solubilité
0.1 M acetate buffer, pH 5.5: soluble
Température de stockage
−20°C
Application
Chitosanase from Sigma has been used for polarized light-stimulated, enzymatic hydrolysis of chitin and chitosan.
Actions biochimiques/physiologiques
Chitosanase catalyzes the endohydrolysis of β-1,4-linkages between D-glucosamine (GlcN-GlcN) residues in chitosan. The enzyme from Streptomyces has been reported to also hydrolyze the GlcNAc-GlcN linkage in partially acetylated chitosan.
Définition de l'unité
One unit will liberate 1.0 μmole of reducing sugar (measured as D-glucosamine equivalents) from chitosan per minute at pH 5.5 at 37 °C.
Forme physique
Solution in 50% glycerol containing 50 mM sodium acetate
Notes préparatoires
Soluble in 10mL of 0.1 M Acetate buffer at pH 5.5
Autres remarques
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
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Les clients ont également consulté
Anna Konieczna-Molenda et al.
Carbohydrate research, 343(18), 3117-3119 (2008-10-01)
Illumination with white linearly polarized light (WLPL) stimulated chitinase and chitosanase in their degradation of chitin and chitosan, respectively. Enzymes were illuminated at room temperature in separate vessels, then admixed in reactors containing polysaccharides. Hydrolysis of chitosan to glucosamine followed
A new reaction for colorimetric determination of carbohydrates.
M Lever
Analytical biochemistry, 47(1), 273-279 (1972-05-01)
T Fukamizo et al.
The Biochemical journal, 311 ( Pt 2), 377-383 (1995-10-15)
Chitosanase was produced by the strain of Streptomyces lividans TK24 bearing the csn gene from Streptomyces sp. N174, and purified by S-Sepharose and Bio-Gel A column chromatography. Partially (25-35%) N-acetylated chitosan was digested by the purified chitosanase, and structures of
Boucher, I., et al.
The Journal of Biological Chemistry, 270, 31-31 (1995)
Ryszard Brzezinski
Bioengineered bugs, 2(4), 226-229 (2011-08-11)
There is a growing interest in chitosanases as enzymatic tools to hydrolyze chitosan into bioactive forms: low molecular weight chitosan (LMWC) or chitosan oligosaccharides (CHOS). However chitosanases are still expensive and methods of large-scale production of these enzymes are not
Articles
Enzymatic Assay of Chitosanase from Streptomyces Species
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