B2904

Bluo-Gal

reagent for selection of recombinant bacterial clones

• Synonyme(s) : 5-Bromo-3-indolyl β-D-galactopyranoside, substrate for β-galactosidase, Bluo-Gal
• Formule empirique (notation de Hill): C₁₄H₁₆BrNO₆
• Numéro CAS: 97753-82-7
• Poids moléculaire : 374.18
• Numéro Beilstein : 1550140
• Numéro MDL: MFCD00063691
• Code UNSPSC : 12352200
• ID de substance PubChem : 24891417
• Nomenclature NACRES : NA.85

Propriétés

• Qualité: for molecular biology
• Niveau de qualité: 200
• Stérilité: non-sterile
• Pureté: ≥98% (HPLC)
• Forme: powder
• Technique(s): nucleic acid detection: suitable
• Solubilité: DMF: soluble
• Adéquation: suitable for substrate for β-galactosidase
• Température de stockage: −20°C
• Chaîne SMILES: OC[C@H]1O[C@@H](Oc2c[nH]c3ccc(Br)cc23)[C@H](O)[C@@H](O)[C@H]1O
• InChI: 1S/C14H16BrNO6/c15-6-1-2-8-7(3-6)9(4-16-8)21-14-13(20)12(19)11(18)10(5-17)22-14/h1-4,10-14,16-20H,5H2/t10-,11+,12+,13-,14-/m1/s1
• Clé InChI: LINMATFDVHBYOS-MBJXGIAVSA-N

Description

Description générale

Bluo-Gal is a chromogenic substrate for β-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. Bluo-Gal is designed to replace X-Gal in blue-white selection of recombinant bacterial colonies with the lac+ phenotype.

Application

Bluo-Gal is suitable for use in selection of recombinant bacterial colonies with the lac⁺ phenotype. Bluo-Gal is suitable for use as β-galactosidase substrate in the staining of skin tissues for electron microscopy analysis, Dictyostelium samples and in the staining of vena cava of adult mice.

Chromogenic substrate suitable for identification of lac⁺ bacterial colonies. An alternative to 5-bromo-4-chloro-3-indolyl β-D-galactopyranoside (X-Gal), producing a darker blue color.

Principe

When Bluo-Gal is hydrolyzed by β-galactosidase, the resulting product will form a blue precipitate. Lac+ colonies grown in the presence of Bluo-Gal turn a deeper blue color more intense than X-Gal, allowing for easy differentiation between lac+ and lac- colonies.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)